Continuous precipitation of IgG from CHO cell culture supernatant in a tubular reactor

Biotechnology Journal
Nikolaus HammerschmidtAlois Jungbauer

Abstract

We successfully transferred a two-stage batch precipitation-based antibody capture step to continuous mode using continuous tubular reactors. The precipitation process solely employs a cheap mineral salt (CaCl2 ) and an organic solvent (ethanol) and could replace the costly protein A capture step in the purification of recombinant antibodies from cell culture supernatant. The time from startup untill attaining steady state conditions was reached in less than 15 minutes and both reactors were operated for several hours at steady state without manual intervention, delivering antibody at a constant yield and purity. An overall yield of > 90 percent, with a host cell protein reduction from 42 777 to 9000 ppm and a DNA reduction from 359 ppm to 7 ppm, could be achieved for the antibody investigated. The precipitated antibody can be dissolved at very high concentrations (> 40 g/L) in numerous buffer systems of various pH and high and low ionic strength, thereby rendering a subsequent concentration or buffer exchange step redundant. This system enables cell culture supernatants with low or high antibody titer to be processed with constant reactor size and without changing any parameters or increasing precipitant consumption. Aggregate...Continue Reading

References

Mar 29, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·R J HarrisA B Chen
Apr 25, 2001·The Journal of Gene Medicine·L PhamK W Peng
Feb 3, 2004·Journal of Chromatography. a·Deborah K Follman, Robert L Fahrner
Dec 6, 2005·Journal of Chromatography. a·Rainer HahnAlois Jungbauer
Nov 23, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Andreas Stein, André Kiesewetter
Jun 15, 2007·Journal of Biotechnology·Ana M AzevedoM Raquel Aires-Barros
Aug 19, 2007·Biochimica Et Biophysica Acta·Andreas Barth
Sep 11, 2007·Journal of Pharmaceutical Sciences·Hongcheng LiuJoanne Sun
Aug 16, 2008·Journal of Chromatography. a·Anne Tscheliessnig, Alois Jungbauer
Dec 21, 2010·Biotechnology Journal·Michael KuczewskiGregory Zarbis-Papastoitsis
Dec 17, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·P A J RosaM R Aires-Barros
Jan 24, 2012·Journal of Chromatography. a·Ekta MahajanBradley Wolk
Apr 11, 2012·Biotechnology and Bioengineering·G R MiesegaesK Brorson
May 30, 2012·Biotechnology Progress·P A Marichal-Gallardo, M M Alvarez
Jun 13, 2012·Biotechnology and Bioengineering·Jens H VogelJennifer Tom
Jun 26, 2012·Biotechnology and Bioengineering·Veena WarikooKonstantin Konstantinov
Jan 22, 2013·Biotechnology and Bioengineering·Benjamin SmejkalDirk Weuster-Botz
Mar 23, 2013·Biotechnology and Bioengineering·Venkatesh Natarajan, Andrew L Zydney
Mar 28, 2013·Biotechnology and Bioengineering·Benjamin SmejkalDariusch Hekmat
Jan 24, 2014·Journal of Pharmaceutical Sciences·Srivalli N TelikepalliDavid B Volkin
Aug 5, 2014·Journal of Biotechnology·Anne TscheliessnigAlois Jungbauer

❮ Previous
Next ❯

Citations

Jun 16, 2015·Journal of Biotechnology·Rahul GodawatVeena Warikoo
Aug 11, 2015·Biotechnology Journal·Guilherme Ferreira, Philippe Jacques
Dec 16, 2016·Biotechnology Progress·Nikhil KatejaAnurag S Rathore
Jun 14, 2018·Biotechnology and Bioengineering·Daniel G GreeneAbraham M Lenhoff
Sep 13, 2018·Biotechnology Journal·Alois Jungbauer
Apr 18, 2020·Biotechnology Journal·Jure SencarAlois Jungbauer
Jul 17, 2018·Journal of Chemical Technology and Biotechnology·Daniel BurgstallerPeter Satzer
Jul 11, 2020·Engineering in Life Sciences·Gregory DutraPeter Satzer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.