Continuous purification of reaction products by micro free-flow electrophoresis enabled by large area deep-UV fluorescence imaging

Analytical and Bioanalytical Chemistry
Simon A PfeifferDetlev Belder

Abstract

Microreactors have gained increasing attention in their application toward continuous micro flow synthesis. An unsolved problem of continuous flow synthesis is the lack of techniques for continuous product purification. Herein, we present a micro free-flow electrophoresis device and accompanying setup that enables the continuous separation and purification of unlabeled organic synthesis products. The system is applied to the separation and purification of triarylmethanes. For imaging of the unlabeled analytes on-chip a novel setup for large area (3.6 cm2) deep ultra violet excitation fluorescence detection was developed. Suitable separation conditions based on low conductivity electrophoresis buffers were devised to purify the product. With the optimized conditions, starting materials and product of the synthesis were well separated (R > 1.2). The separation was found to be very stable with relative standard deviations of the peak positions smaller than 3.5% over 15 min. The stable conditions enabled collection of the separated compounds, and purity of the product fraction was confirmed using capillary electrophoresis and mass spectrometry. This result demonstrates the great potential of free-flow electrophoresis as a technique...Continue Reading

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Citations

Jun 27, 2020·Lab on a Chip·Tom ScheidtTuomas P J Knowles
Nov 30, 2018·Chemical Communications : Chem Comm·Faith M Akwi, Paul Watts
Sep 13, 2020·Analytical and Bioanalytical Chemistry·Chris PrüfertHans-Gerd Löhmannsröben
Jul 14, 2021·Analytical Chemistry·Sven KochmannSergey N Krylov
Jul 4, 2018·Analytical Chemistry·Sven Kochmann, Sergey N Krylov
Nov 9, 2019·Analytical Chemistry·Courtney J KristoffLisa A Holland
Jan 29, 2020·Analytical Chemistry·Nikita A IvanovSergey N Krylov

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