Aug 1, 1976

Continuous regeneration of NAD(P)+ by flavins covalently bound to sepharose

Biotechnology and Bioengineering
M O MånssonK Mosbach

Abstract

Various flavins, FMN, FAD, and acriflavin, were immobilized to Sepharose using several different coupling methods. The only product stable enough to permit extended studies was acriflavin coupled to epoxy-substituted Sepharose. The nonenzymic oxidizing capacity towards NAD(P) H was investigated and a 25% specific activity, compared to that of free acriflavin, was observed. The reduced acriflavin was immediately auto-reoxidized in air and could thus be reused. It was shown that acriflavin-Sepharose preparations function as NAD(P)H oxidizing agents in a number of different dehydrogenase systems including lactate dehydrogenase (LDH), alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), alanine dehydrogenase (alaDH), and glutamate dehydrogenase (GDH). The amount of expensive coenzyme necessary for high product formation of such systems was thereby markedly reduced.

Mentioned in this Paper

Oxidase
Sepharose
NADP
Lactate Dehydrogenase Deficiency Type A
Epoxy Resins
Oxidation-Reduction
Flavins
Acriflavine
GLUD1
Flavin Mononucleotide Monosodium Salt, Dihydrate

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