Continuous sheath-free separation of drug-treated human fungal pathogen Cryptococcus neoformans by morphology in biocompatible polymer solutions

Electrophoresis
Di LiXiangchun Xuan

Abstract

Cryptococcal meningitis caused by Cryptococcus neoformans is the leading cause of fungal central nervous system infections. Current antifungal treatments for cryptococcal infections are inadequate partly due to the occurrence of drug resistance. Recent studies indicate that the treatment of the azole drug fluconazole changes the morphology of C. neoformans to form enlarged "multimeras" that consist of three or more connected cells/buds. To analyze if these multimeric cells are a prerequisite for C. neoformans to acquire drug resistance, a tool capable of separating them from normal cells is critical. We extend our recently demonstrated sheath-free elasto-inertial particle separation technique to fractionate drug-treated C. neoformans cells by morphology in biocompatible polymer solutions. The separation performance is evaluated for both multimeric and normal cells in terms of three dimensionless metrics: efficiency, purity, and enrichment ratio. The effects of flow rate, polymer concentration, and microchannel height on cell separation are studied.

References

Dec 1, 1993·Journal of Clinical Microbiology·J R PerfectC L Beiser
May 15, 2004·Science·Lotien Richard HuangJames C Sturm
Mar 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Julie A Champion, Samir Mitragotri
Aug 19, 2007·Electrophoresis·Mario Urdaneta, Elisabeth Smela
Apr 28, 2009·Physical Review Letters·Dino Di CarloMehmet Toner
Oct 14, 2009·Lab on a Chip·Dino Di Carlo
Oct 22, 2009·Cell Cycle·Sophie G Martin
Dec 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ayse R KoseHur Koser
Mar 20, 2010·Electrophoresis·Junjie ZhuXiangchun Xuan
Apr 27, 2010·Analytical and Bioanalytical Chemistry·Daniel R GossettDino Di Carlo
Feb 14, 2012·Lab on a Chip·Jason P BeechJonas O Tegenfeldt
Jan 10, 2014·Biomicrofluidics·A KarimiA M Ardekani
Apr 23, 2014·Biomicrofluidics·John DuboseXiangchun Xuan
Aug 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Vikram JadhaoMonica Olvera de la Cruz
May 26, 2015·Analytical Chemistry·Xinyu Lu, Xiangchun Xuan
Oct 28, 2015·Analytical Chemistry·Xinyu Lu, Xiangchun Xuan
Dec 22, 2016·Lab on a Chip·Ran ZhouCheng Wang
Apr 17, 2017·Journal of Colloid and Interface Science·Xinyu LuXiangchun Xuan
Nov 21, 2017·Biomicrofluidics·Qi ChenXiangchun Xuan

❮ Previous
Next ❯

Citations

Oct 28, 2019·Biotechnology and Bioengineering·Mohammad K D ManshadiAmir Sanati-Nezhad

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Central Nervous System Fungal Infections

Central nervous system fungal infections are rare and typically occur in immunocompromised patients, such as those with HIV infection or transplant recipients. Discover the latest research on central nervous system fungal infections here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Related Papers

Transplant Infectious Disease : an Official Journal of the Transplantation Society
Aviv V PudipeddiSimone I Strasser
North American Journal of Medical Sciences
Veronica A Gaona-Flores
Journal of Laboratory Physicians
Rm Saldanha DominicShrikala Baliga
Vojnosanitetski pregled. Military-medical and pharmaceutical review
M DokićJ Dimitrijević
© 2022 Meta ULC. All rights reserved