PMID: 1368563Jun 1, 1990

Continuous synthesis of a tripeptide by successive condensation and transesterification catalyzed by two immobilized proteinases in organic solvent

Agricultural and Biological Chemistry
Y KimuraR Matsuno

Abstract

The tripeptide Z-GlyPheLeuNH2 was continuously synthesized in a high yield from three amino acid derivatives, Z-Gly, PheOMe, and LeuNH2, by immobilized thermolysin (IMT) and immobilized alpha-chymotrypsin (IMC) in an organic solvent, ethyl acetate. The optimal conditions for the synthesis of Z-GlyPheOMe were established theoretically. The yield of Z-GlyPheOMe with IMT in ethyl acetate saturated with buffer was more than 88% after continuous synthesis for 116 hr. The optimal conditions for the synthesis of Z-GlyPheLeuNH2 from Z-GlyPheOMe and LeuNH2 by IMC through transesterification was established in batch reaction experiments. When the concentration of water in the reaction solution was 17-20 microliters/ml, the activity of IMC was highest. The equilibrium between the water concentration in the reaction solution and that in the resin used for enzyme immobilization depended on the resin and was not affected by the presence of the enzyme immobilized. Z-GlyPheLeuNH2 was synthesized from Z-GlyPheOMe and LeuNH2 with a yield of 100%, by continuous reaction for 160 hr. The reactor for synthesis of this tripeptide was efficient and stable because of the use of transesterification and the choice of an appropriate organic solvent. The s...Continue Reading

Citations

Oct 20, 2005·Journal of Bioscience and Bioengineering·M MiyanagaK Nakanishi
Sep 1, 2005·Applied Microbiology and Biotechnology·Dinesh Kumar, Tek Chand Bhalla
Feb 15, 1996·Enzyme and Microbial Technology·I GillE N Vulfson
May 1, 1994·Biotechnology and Bioengineering·T NagayasuK Nakanishi
May 1, 1994·Biotechnology and Bioengineering·T NagayasuK Nakanishi

Related Concepts

Ethyl acetate
Acetic Acids
Avazyme
Hydrogen-Ion Concentration
Polypeptides
Thermolysin

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