Continuous uroflow cystometry in the urethane-anesthetized mouse.

Neurourology and Urodynamics
Phillip P Smith, George A Kuchel

Abstract

In vivo animal cystometry represents an accepted methodology for the study of lower urinary tract physiology. A particular advantage of the mouse model is the availability of genetically modified strains, offering the possibility of linking individual genes to relevant physiological events. However, small voided volumes complicate the ability to obtain reliable pressure-flow data by gravimetric methods, due to non-continuous drop formation and release during voiding. We investigated the feasibility of a simple non-gravimetric continuous urine collection system during cystometry under urethane anesthesia, and compared urethane-anesthetized with awake cystometry. Cystometry was performed in awake and urethane-anesthetized female mice using a suprapubic tube. A simple, novel non-gravimetric method of urine collection was used in urethane-anesthetized animals to assess voided volume and permit flow rate calculations. Pressure and time-related variables were compared between groups. Voided urine collection appears to be complete and continuous in this model. Mean voided volume was 0.09 ± 0.020 ml, with an average flow rate of 0.029 ± 0.007 ml/sec. Urethane anesthesia delayed cystometric pressure/volume responses. However, micturitio...Continue Reading

References

Jul 1, 1990·Journal of the Autonomic Nervous System·C A Maggi, B Conte
Mar 1, 1997·The Journal of Urology·R S SutherlandD S Bredt
Nov 24, 1999·The American Journal of Physiology·J le Feber, E van Asselt
Dec 22, 1999·Neurourology and Urodynamics·S Matsuura, J W Downie
Aug 18, 2001·Life Sciences·T W Cannon, M S Damaser
Oct 17, 2001·Journal of the American Veterinary Medical Association·R Schwiebert
Jun 26, 2004·BJU International·Tomi StrengAntti Talo
Jun 11, 2005·The Journal of Urology·Qin ChenTadaichi Kitamura
Oct 18, 2005·BJU International·Tomi K StrengRisto Santti
Apr 26, 2006·Neurourology and Urodynamics·Chih-Wei PengChen-Li Cheng
Jan 6, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Paul C DolberJerome P Reiter
Dec 9, 2008·Molecular and Cellular Endocrinology·Taija SaloniemiMatti Poutanen

❮ Previous
Next ❯

Citations

Dec 30, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Phillip P SmithGeorge A Kuchel
Aug 22, 2012·Journal of Visualized Experiments : JoVE·Duong D TuCarlos R Estrada
Sep 23, 2014·International Urology and Nephrology·Pradeep TyagiNaoki Yoshimura
Dec 3, 2014·Computers in Biology and Medicine·Di LiuFeng Pan
May 23, 2015·Neurourology and Urodynamics·Brandon K LaPalloJonathan S Carp
Nov 1, 2011·Neurourology and Urodynamics·Phillip P SmithGeorge A Kuchel
Jun 11, 2011·Neurourology and Urodynamics·Karl-Erik AnderssonClaudius Füllhase
Aug 15, 2014·BJU International·Phillip P SmithRichard Simon
Jul 2, 2016·Laboratory Animals·Els van AsseltRon van Mastrigt
Jan 18, 2017·Scientific Reports·Jae Hong ParkJun-Kyo Francis Suh
Jul 29, 2016·American Journal of Physiology. Renal Physiology·Leonie DurninVioleta N Mutafova-Yambolieva
Aug 9, 2018·Neurourology and Urodynamics·Mariana G de OliveiraEdson Antunes
Apr 11, 2014·American Journal of Physiology. Renal Physiology·Weiqun YuMark L Zeidel
Oct 22, 2020·Neurourology and Urodynamics·William GibsonAdrian Wagg
Jan 16, 2020·European Urology Focus·Andrea M SartoriMartin E Schwab
Mar 20, 2021·Neurourology and Urodynamics·Ramalakshmi Ramasamy, Phillip P Smith
Jul 25, 2021·Science Advances·Jan FrankenGreetje Vande Velde

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.