Contortrostatin, a homodimeric snake venom disintegrin, is a potent inhibitor of osteoclast attachment

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
B MercerC Minkin

Abstract

Disintegrins are small disulfide-rich proteins containing an Arg-Gly-Asp (RGD) sequence near their carboxyl terminus. These polypeptides inhibit binding of adhesion molecules to their receptors (integrins) on the surface of cells. Osteoclasts express integrins, heterodimeric cell surface adhesion receptors, that have been shown to be involved in interactions with the extracellular matrix (ECM), including attachment to bone and bone resorption. It has recently been shown that disintegrins effectively inhibit attachment of osteoclasts to components of the ECM and also disrupt osteoclast-mediated bone resorption. Here we characterize the effects of contortrostatin (CTS), a novel homodimeric snake venom disintegrin, on osteoclast attachment. Plastic dishes coated with CTS were able to support osteoclast attachment with a high affinity (EC50,CTS = 86 +/- 6.7 nM) similar to that of vitronectin (VTN; EC50,VTN = 80 +/- 20 nM). Further, CTS was observed to inhibit completely osteoclast attachment to fetal bovine serum (FBS; IC50,FBS = 0.36 +/- 0.04 nM) and VTN (IC50,VTN = 0.85 +/- 0.13 nM). We used monoclonal antibodies directed against the beta1 (monoclonal antibody [MAb] CD29) and beta3 (MAb F11) integrin subunits to explore the mecha...Continue Reading

References

Mar 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M H HelfrichM A Horton
Mar 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M H HelfrichM A Horton
Jan 1, 1991·The Journal of Clinical Investigation·E Ruoslahti
Oct 1, 1990·The Journal of Cell Biology·M SatoR J Gould
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M S DennisR A Lazarus
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R PytelaE Ruoslahti
Dec 1, 1989·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M A Horton, J Davies
Oct 23, 1987·Science·E Ruoslahti, M D Pierschbacher
Feb 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M A HortonM H Helfrich
Sep 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M SatoR J Gould
Mar 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K L KingS C Kukreja

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Citations

Feb 27, 2010·Critical Reviews in Biochemistry and Molecular Biology·Monika Gooz
Jan 22, 2005·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Arkadiusz SurazyńskiJerzy Pałka
Oct 9, 2016·Cell Biochemistry and Biophysics·Bilu XiangHaiyang Yu
Jul 15, 2020·Biochemical Pharmacology·Axel H SchönthalFrancis S Markland
Jan 7, 2000·Biochemical and Biophysical Research Communications·Q ZhouF S Markland
Sep 15, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Xing-Ding ZhouYu-Liang Xiong
Nov 6, 2008·Toxicon : Official Journal of the International Society on Toxinology·Antony GomesAparna Gomes

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