Contour and persistence length of Corynebacterium diphtheriae pili by atomic force microscopy.

European Biophysics Journal : EBJ
Johannes RheinlaenderTilman E Schäffer

Abstract

Many bacteria are characterized by nanoscale ultrastructures, for example S-layers, flagella, fimbriae, or pili. The last two are especially important for attachment to different abiotic and biotic surfaces and for host-pathogen interactions. In this study, we investigated the geometric and elastic properties of pili of different Corynebacterium diphtheriae strains by atomic force microscopy (AFM). We performed quantitative contour-length analysis of bacterial pili and found that the visible contour length of the pili can be described by a log-normal distribution. Our data revealed significant strain-specific variations in the mean visible contour length of the pili, ranging from 260 to 1,590 nm. To estimate their full contour length, which is not directly accessible from the AFM images, we developed a simple correction model. Using this model, we determined the mean full contour length as 510-2,060 nm. To obtain the persistence length we used two different methods of analysis, one based on the end-to-end distance of the pili and one based on the bending angles of short segments. In comparison, the bending angle analysis proved to be more precise and resulted in persistence lengths in the narrow range of 220-280 nm, with no sig...Continue Reading

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Citations

Jun 1, 2013·ISRN Microbiology·Andreas Burkovski
Dec 3, 2013·Biochimica Et Biophysica Acta·Flavien PilletEtienne Dague
Dec 9, 2020·Nanoscale·Audrey BeaussartSofiane El-Kirat-Chatel
Apr 22, 2020·ACS Applied Materials & Interfaces·Ibrahima DraméEtienne Dague

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