Contractile tension and beating rates of self-exciting monolayers and 3D-tissue constructs of neonatal rat cardiomyocytes.

Medical & Biological Engineering & Computing
P T LinderA Temiz Artmann

Abstract

The CellDrum technology (The term 'CellDrum technology' includes a couple of slightly different technological setups for measuring lateral mechanical tension in various types of cell monolayers or 3D-tissue constructs) was designed to quantify the contraction rate and mechanical tension of self-exciting cardiac myocytes. Cells were grown either within flexible, circular collagen gels or as monolayer on top of respective 1-mum thin silicone membranes. Membrane and cells were bulged outwards by air pressure. This biaxial strain distribution is rather similar the beating, blood-filled heart. The setup allowed presetting the mechanical residual stress level externally by adjusting the centre deflection, thus, mimicking hypertension in vitro. Tension was measured as oscillating differential pressure change between chamber and environment. A 0.5-mm thick collagen-cardiac myocyte tissue construct induced after 2 days of culturing (initial cell density 2 x 10(4) cells/ml), a mechanical tension of 1.62 +/- 0.17 microN/mm(2). Mechanical load is an important growth regulator in the developing heart, and the orientation and alignment of cardiomyocytes is stress sensitive. Therefore, it was necessary to develop the CellDrum technology with ...Continue Reading

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Citations

Apr 24, 2012·Journal of Pharmacological and Toxicological Methods·Anna GrosbergKevin Kit Parker
May 23, 2012·Journal of Bioscience and Bioengineering·Eylem Kurulgan DemirciAysegul Temiz Artmann
Sep 7, 2012·Biotechnology and Bioengineering·Emerson J De SouzaTaher Saif
Apr 14, 2016·Biophysical Journal·Meghan B KnightAnna Grosberg
Jun 29, 2013·Lab on a Chip·Ashutosh AgarwalKevin Kit Parker
Mar 26, 2015·Journal of Biomechanical Engineering·Ralf FrotscherManfred Staat
Apr 22, 2020·Annals of Biomedical Engineering·Viktor A BalashovKonstantin I Agladze

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