Contractility in vitro and mitochondrial response in small and large rabbit bowel after anastomosis

World Journal of Surgery
Mario TestiniG Palasciano

Abstract

Leakage of a large bowel anastomosis remains the most serious postoperative complication in gastrointestinal surgery. In a recent experimental study we found that surgically induced hypoxia resulted in more derangement of a variety of biochemical markers in the large bowel (LB) than in the small bowel (SB). We explored the question of whether spontaneous and agonist-induced contractility of SB and LB muscle strips was influenced by surgical procedures and how contractility was related to energetic oxidative metabolism capacity in smooth muscle mitochondria. Sixty male New Zealand rabbits were operated on under general anesthesia. Segments of ileum and colon were resected from each rabbit, and an end-to-end anastomosis was constructed. A representative subset of segments from SB (n = 14) and LB (n = 14) at time 0 was used as controls. Tracts containing an anastomosis were resected at days 2, 7, and 14 after operation. At each time point, 20 segments adjacent to the anastomosis of both SB and LB were used for tensiometric and biochemical studies. Tensiometric studies demonstrated modifications in the smooth muscle function at both the acute and chronic stages with intestinal inflammation that may contribute to surgical stress-ass...Continue Reading

Citations

Oct 17, 2003·World Journal of Gastroenterology : WJG·Mario TestiniLuigi Greco
Mar 1, 2012·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Hiroyuki FujimotoHikaru Suzuki

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