PMID: 809571Aug 1, 1975

Contractions induced by a calcium-triggered release of calcium from the sarcoplasmic reticulum of single skinned cardiac cells

The Journal of Physiology
A Fabiato, F Fabiato

Abstract

1. Fragments of single cardiac cells were obtained by homogenization of ventricular tissue from adult rats. Remaining pieces of sacrolemma were removed by micro-dissection. Tension was recorded from the ends of the skinned (sarcolemma-free) cells with a photodiode force transducer. 2. In the presence of a strong buffering of the free [Ca2+] with 4-0 mM total EGTA, a tonic tension was obtained that increased according to t sigmoid curve when the free ([Ca2+] was increased from 10(-6-75)M to 10(-5-0)M. This curve was not modified by the destruction of the sarcoplasmic reticulum (SR) by the detergent Brij 58. Therefore, the tonic tension corresponded to the direct effect of the free [Ca2+] present in the buffer on the myofilaments. 3. In the presence of a slight buffering of the free [Ca2+] with 0-050 mM total EGTA, cyclic contractions were observed that were attributed to cyclic releases and re-sequestrations of Ca2+ by the SR. The absence of effect of azide and ruthenium red on the cyclic contractions obtained at a free [Ca2+] lower than 10(-6-50)M demonstrated that the mitochondria played no role in the triggering of these contractions. 4. Cyclic contractions were induced by a slight variation of free [Ca2+] in the buffer from ...Continue Reading

References

Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y WuM E Anderson
Mar 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Shi Qiang WangHeping Cheng
Mar 1, 1984·Medical & Biological Engineering & Computing·P A WieringaJ D Laird
Apr 14, 2011·Pflügers Archiv : European journal of physiology·Jolanda van der Velden
Mar 15, 1981·Experientia·M S GaideA L Bassett
Apr 4, 2006·Acta Biotheoretica·Audrey Pustoc'hPhilippe Tracqui
Jan 1, 1995·Journal of Electrocardiology·I GussakB R Chaitman
Mar 21, 2012·The Journal of Physiological Sciences : JPS·Etsuko TanakaSatoshi Kurihara
Nov 1, 1993·The Journal of Pharmacy and Pharmacology·S I Sharif
Sep 1, 1996·Pflügers Archiv : European journal of physiology·A MinajevaV Veksler
Aug 1, 1990·Pflügers Archiv : European journal of physiology·F V BrozovichK G Morgan
Oct 31, 2012·Cellular and Molecular Life Sciences : CMLS·Marta GaburjákováMuthu Periasamy
Sep 1, 1995·Journal of Biomechanics·W F BluhmW Y Lew
Jan 1, 1985·Progress in Biophysics and Molecular Biology·J CailléO Rougier
Jan 1, 1979·Progress in Biophysics and Molecular Biology·R A Chapman
May 15, 2004·Progress in Biophysics and Molecular Biology·Jose L PuglisiDonald M Bers
Mar 1, 2006·Progress in Biophysics and Molecular Biology·Fabien BretteLaurent Sallé
Aug 31, 1990·Biophysical Chemistry·A Goldbeter, G Dupont
Oct 3, 2003·Biochemical and Biophysical Research Communications·Xu Dong LiaoShi Qiang Wang
Jul 11, 2006·Wiener medizinische Wochenschrift·Klara BrixiusRobert H G Schwinger
Feb 12, 2005·Clinical Cardiology·Heinz-Gerd Zimmer
Apr 1, 1996·Clinical Cardiology·V DucceschiA Iacono
Jul 1, 2010·Cardiovascular Research·Michel Pucéat
May 1, 1996·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·S YanagidaM M Pike
Jan 1, 1992·Bulletin of Mathematical Biology·A Y WongJ B Bassingthwaighthe
Mar 1, 1993·Bulletin of Mathematical Biology·J SneydD Clapham
Aug 2, 2012·The Journal of General Physiology·Barbora TencerováMarta Gaburjáková
Apr 1, 1994·Molecular and Cellular Biochemistry·R Ventura-ClapierJ A Hoerter
Feb 10, 2009·Molecular and Cellular Biochemistry·João Paulo L DaherDaniel Weinreich
Mar 17, 2012·Pediatric Cardiology·Michela FaggioniBjörn C Knollmann

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