Contradictory roles of Porphyromonas gingivalis gingipains in caspase-1 activation

Cellular Microbiology
Young-Jung JungBong-Kyu Choi

Abstract

Porphyromonas gingivalis utilizes its major proteases, Arg gingipains (RgpA and RgpB) and Lys gingipain (Kgp), for dysregulation of host immune systems. The aim of this study was to investigate the roles of gingipains in caspase-1 activation and its sequelae in P. gingivalis-infected macrophages. Infection with P. gingivalis at low multiplicity of infections (MOIs), but not at high MOIs, resulted in low levels of interleukin-1β and lactate dehydrogenase without detectable active caspase-1 in the culture supernatants. The proteins released from caspase-1-activated cells were rapidly degraded by gingipains. However, P. gingivalis with gingipains induced higher intracellular caspase-1 activity in the infected cells than the gingipain-null mutant, which was associated with ATP release from the infected cells. In addition, growing the gingipain-null mutant with gingipains enhanced caspase-1 activation by the mutant. In contrast, inhibition of the protease activity of Kgp or Rgps increased the caspase-1-activating potential of wild-type P. gingivalis, indicating an inhibitory effect of the collaborative action of Kgp and Rgps. These results illuminate the contradictory roles of gingipains in the manipulation of host defence systems b...Continue Reading

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Citations

Jul 13, 2016·Mediators of Inflammation·P C Carvalho-FilhoS C Trindade
Aug 15, 2020·Molecular Oral Microbiology·Hye-Kyung JunBong-Kyu Choi
Jul 26, 2019·Mediators of Inflammation·Kívia Queiroz de AndradeRobson Coutinho-Silva
Aug 6, 2019·Frontiers in Cellular and Infection Microbiology·Lu JiaYi Liu

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