Contrast-Enhanced Microtomographic Characterisation of Vessels in Native Bone and Engineered Vascularised Grafts Using Ink-Gelatin Perfusion and Phosphotungstic Acid

Contrast Media & Molecular Imaging
Sarah SutterDirk J Schaefer

Abstract

Bone ischemia and necrosis are challenging to treat, requiring investigation of native and engineered bone revascularisation processes through advanced imaging techniques. This study demonstrates an experimental two-step method for precise bone and vessel analysis in native bones or vascularised bone grafts using X-ray microtomography (μCT), without interfering with further histological processing. Distally ligated epigastric arteries or veins of 6 nude rats were inserted in central channels of porous hydroxyapatite cylinders and these pedicled grafts were implanted subcutaneously. One week later, the rats were perfused with ink-gelatin and euthanised and the femurs, tibias, and grafts were explanted. Samples were scanned using μCT, decalcified, incubated with phosphotungstic acid (PTA) for contrast enhancement, rescanned, and processed histologically. Contrast-enhanced μCT displayed the course and branching of native bone vessels. Histologically, both central (-17%) and epiphyseal vessels (-58%) appeared smaller than in μCT scans. Hydroxyapatite cylinders were thoroughly vascularised but did not display bone formation. Grafts with a central artery had more (+58%) and smaller (-52%) vessel branches compared to grafts with a vei...Continue Reading

References

Mar 1, 1969·The Journal of Cell Biology·L Silverman, D Glick
Jan 20, 2004·Radiographics : a Review Publication of the Radiological Society of North America, Inc·Tobias BoskampPeter Mildenberger
Feb 22, 2007·AJR. American Journal of Roentgenology·Silke GrabherrRichard Dirnhofer
Jul 29, 2008·Tissue Engineering. Part B, Reviews·Simon YoungAntonios G Mikos
Dec 20, 2008·The American Journal of Pathology·Thomas Levin AndersenJean-Marie Delaisse
Feb 25, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Brian D Metscher
Feb 27, 2009·IEEE Transactions on Medical Imaging·Dong-Goo KangJong Beom Ra
Dec 4, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Jia FeiLafage-Proust Marie-Hélène
Jun 10, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Mary L BouxseinRalph Müller
Sep 8, 2010·Bone·Michael DoubeSandra J Shefelbine
Jan 1, 2009·Psychological Science in the Public Interest : a Journal of the American Psychological Society·Timothy B BakerVarda Shoham
Mar 30, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wouter F WillemsAllen T Bishop
May 3, 2011·Clinical Orthopaedics and Related Research·Wouter F WillemsThomas Kremer
Jul 5, 2011·Acta Biomaterialia·Volker AltAlexander C Langheinrich
Mar 30, 2012·Annali Dell'Istituto Superiore Di Sanità·Andrea BarbettaMariella Dentini
Jul 17, 2012·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·M JakobI Martin
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Jun 27, 2013·Tissue Engineering. Part C, Methods·Ioannis PapantoniouGreet Kerckhofs
Aug 1, 2012·Materials Science & Engineering. C, Materials for Biological Applications·Qing-shan DongTian-qiu Mao
Jan 15, 2014·Orthopaedics & Traumatology, Surgery & Research : OTSR·P RossetP Layrolle
Mar 22, 2014·Eklem hastalıkları ve cerrahisi = Joint diseases & related surgery·Nurzat ElmalıHaldun Topgül
Apr 3, 2014·Journal of Orthopaedic Surgery and Research·Talal Al-JabriPeter Giannoudis
Sep 10, 2014·BMC Musculoskeletal Disorders·Svitlana GayetskyyKlaus Engelke
Sep 28, 2014·Bone·Adam PapadimitropoulosIvan Martin
Apr 11, 2015·BoneKEy Reports·Marie-Hélène Lafage-ProustLaurence Vico
Jun 9, 2015·International Journal of Molecular Sciences·Alexandre KaempfenDirk J Schaefer
Jul 3, 2015·Medical Physics·Fengjun ZhaoFeng Cao
Nov 11, 2016·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Hong-Ming XuXiang-Yang Wang

❮ Previous
Next ❯

Citations

Sep 22, 2018·Current Protocols in Mouse Biology·Andrew BleasePaul K Potter
Feb 21, 2019·Microscopy Research and Technique·Mümin Mehmet KoçAsa H Barber
Oct 14, 2020·Journal of Tissue Engineering and Regenerative Medicine·Tarek IsmailArnaud Scherberich

❮ Previous
Next ❯

Methods Mentioned

BETA
sedation
X-ray
dissection
fluorescence microscopy

Software Mentioned

GraphPad
GraphPad Prism
VG Studio MAX
phoenix
ImageJ
Excel
GE
BoneJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nordisk medicin
K B Sorensen
European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery
F C Brochado NetoM Albers
© 2022 Meta ULC. All rights reserved