Contrast-enhanced ultrasound measurement of microvascular perfusion relevant to nutrient and hormone delivery in skeletal muscle: a model study in vitro

Microvascular Research
Renee M RossStephen Rattigan

Abstract

Contrast-enhanced ultrasound (CEU) has been used to measure muscle microvascular perfusion in vivo in response to exercise and insulin. In the present study we address whether CEU measurement of capillary volume is influenced by bulk flow and if measured capillary filling rate allows discrimination of different flow pattern changes within muscle. Three in vitro models were used: (i) bulk flow rate was varied within a single length of capillary tubing; (ii) at constant bulk flow, capillary volume was increased 3-fold by joining lengths of capillary in series, and compared to a single length; and (iii) at constant bulk flow, capillary volume was increased by sharing flow between a number of lengths of identical capillaries in parallel. The contrast medium for CEU was gas-filled albumin microbubbles. Pulsing interval (time) versus acoustic-intensity curves were constructed and from these, capillary volume and capillary filling rate were calculated. CEU estimates of capillary volume were not affected by changes in bulk flow. Furthermore, as CEU estimates of capillary volume increased, measures of capillary filling rate decreased, regardless of whether capillaries were connected in series or parallel. Therefore, CEU can detect a cha...Continue Reading

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Citations

Jul 10, 2008·American Journal of Physiology. Endocrinology and Metabolism·Michael G Clark
Aug 9, 2015·Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine·Dmitry GrishenkovBirgitta Janerot-Sjoberg
Jun 29, 2017·Ultrasound in Medicine & Biology·Sarah J BlackwoodStephen Rattigan
Dec 24, 2019·Clinical and Experimental Pharmacology & Physiology·Katherine M Roberts-ThomsonMichelle A Keske
Oct 13, 2019·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Thorbjorn AkerstromChristopher G Ellis
May 31, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Kim A SjøbergBente Kiens

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