Contrasting Effects of Adipokines on the Cytokine Production by Primary Human Bronchial Epithelial Cells: Inhibitory Effects of Adiponectin

Frontiers in Pharmacology
Hélène SalvatorPhilippe Devillier

Abstract

Obesity is associated with an elevated risk of respiratory infections and inflammatory lung diseases. The objective was to investigate (i) the effects of adipokines (adiponectin (APN), leptin, chemerin, and visfatin) on the production of cytokines by unstimulated and poly(I:C)- and TNF-α-activated human primary bronchial epithelial cells (hBECs), (ii) the cells' expression of the APN receptors (AdipoR1 and AdipoR2), and (iii) the cells' production of APN. The hBECs were isolated from patients undergoing surgery for lung carcinoma. The cells were then cultured with human recombinant adipokines in the absence or presence of TNF-α or poly(I:C) for 24 h. Supernatant levels of cytokines (IL-6, CCL2, CCL5, CCL20, CXCL1, CXCL8) and APN were measured using ELISAs. The mRNA levels of AdipoR1 and AdipoR2 in hBECs were determined using a real-time quantitative PCR. Of the four adipokines tested, only APN significantly influenced the basal production and the TNF-α poly(I:C)-induced production of cytokines by hBECs. APN (3-30 µg.ml-1) was associated with greater basal production of IL-6, CCL20, and CXCL8, lower basal production of CCL2 and CXCL1 and no difference in CCL5 production. APN inhibited the poly(I:C)-induced production of these fi...Continue Reading

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Citations

Jun 13, 2020·European Journal of Clinical Investigation·Adrian PostRobin P F Dullaart
Jul 15, 2020·Clinical Drug Investigation·Antonella FioravantiSara Cheleschi
Jul 15, 2020·Clinical Drug Investigation·Bin Liu, Zhigang Qi
May 12, 2020·Frontiers in Public Health·Philip B Maffetone, Paul B Laursen
Jul 12, 2020·Journal of Clinical Medicine·Amira Sayed KhanNaim Akhtar Khan
Sep 18, 2020·Current Opinion in Clinical Nutrition and Metabolic Care·Hans K Biesalski
Mar 16, 2021·Frontiers in Physiology·Leandro Ribeiro CostaAna Cláudia Garcia de Oliveira Duarte
Jul 27, 2021·Frontiers in Cell and Developmental Biology·Marjan Nouri-KeshtkarYaser Tahamtani
Aug 28, 2021·International Journal of Molecular Sciences·Marina Ruxandra OteleaAgripina Rascu
Sep 14, 2021·Frontiers in Pharmacology·Hélène SalvatorPhilippe Devillier

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Methods Mentioned

BETA
surgical resection
dissection
FCS
ELISAs
Assay
electrophoresis
PCR
confocal microscopy
transfection

Software Mentioned

GraphPad Prism
GraphPad

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