Contrasting effects of EWI proteins, integrins, and protein palmitoylation on cell surface CD9 organization

The Journal of Biological Chemistry
Xiuwei H YangMartin E Hemler

Abstract

CD9, a tetraspanin protein, makes crucial contributions to sperm egg fusion, other cellular fusions, epidermal growth factor receptor signaling, cell motility, and tumor suppression. Here we characterize a low affinity anti-CD9 antibody, C9BB, which binds preferentially to homoclustered CD9. Using mAb C9BB as a tool, we show that cell surface CD9 homoclustering is promoted by expression of alpha3beta1 and alpha6beta4 integrins and by palmitoylation of the CD9 and beta4 proteins. Conversely, CD9 is shifted toward heteroclusters upon expression of CD9 partner proteins (EWI-2 and EWI-F) or other tetraspanins, or upon ablation of CD9 palmitoylation. Furthermore, unpalmitoylated CD9 showed enhanced EWI-2 association, thereby demonstrating a previously unappreciated role for tetraspanin palmitoylation, and underscoring how depalmitoylation and EWI-2 association may collaborate to shift CD9 from homo- to heteroclusters. In conclusion, we have used a novel molecular probe (mAb C9BB) to demonstrate the existence of multiple types of CD9 complex on the cell surface. A shift from homo- to heteroclustered CD9 may be functionally significant because the latter was especially obvious on malignant epithelial tumor cells. Hence, because of its...Continue Reading

References

Feb 1, 1990·British Journal of Haematology·R E WorthingtonC Boucheix
Mar 1, 1972·Immunochemistry·D M Crothers, H Metzger
Oct 7, 1998·International Journal of Cancer. Journal International Du Cancer·M ShoM Miyake
May 14, 1999·International Immunology·K DrbalV Horejsí
Jan 15, 2000·Science·F Le NaourC Boucheix
Nov 23, 2000·The Journal of Biological Chemistry·C S StippM E Hemler
Mar 30, 2001·The Journal of Biological Chemistry·S CharrinE Rubinstein
Aug 16, 2001·The Journal of Biological Chemistry·C S StippM E Hemler
Apr 18, 2002·FEBS Letters·Stéphanie CharrinEric Rubinstein
Nov 2, 2002·The Journal of Biological Chemistry·Maria Dolores Gutierrez-LopezCarlos Cabanas
Apr 23, 2003·Biochemical and Biophysical Research Communications·Stéphanie CharrinEric Rubinstein
Apr 24, 2003·The Biochemical Journal·Stéphanie CharrinEric Rubinstein
Jun 11, 2003·The Journal of Cell Biology·Yoshito TakedaEisuke Mekada
Aug 26, 2003·European Journal of Immunology·Stéphanie CharrinEric Rubinstein
Sep 11, 2003·Head & Neck·Boban M ErovicCsilla Neuchrist
Dec 10, 2003·The Journal of Cell Biology·Christopher S StippMartin E Hemler
Jan 7, 2004·The Journal of Biological Chemistry·Wei Tang, Martin E Hemler
Jan 13, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tomoko IshibashiHiroko Baba
Apr 9, 2004·Blood·Tatiana V KolesnikovaMartin E Hemler
May 27, 2004·The Journal of Biological Chemistry·Anu CherukuriSusan K Pierce
Jun 18, 2004·Molecular Biology of the Cell·Wei TangMartin E Hemler

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Citations

Dec 17, 2008·Nature Reviews. Cancer·Margot Zöller
Feb 8, 2014·Nature Reviews. Cancer·Martin E Hemler
Sep 2, 2008·Nature Reviews. Drug Discovery·Martin E Hemler
Sep 19, 2009·The Journal of Biological Chemistry·Stéphanie CharrinOlivier Silvie
Feb 24, 2011·The Journal of Biological Chemistry·Claire MontpellierLaurence Cocquerel
May 30, 2008·Molecular Biology of the Cell·Chandan SharmaMartin E Hemler
Jul 21, 2011·Journal of Cell Science·Hong-Xing WangMartin E Hemler
Apr 3, 2008·PloS One·Vera Rocha-PeruginiLaurence Cocquerel
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Juwen ShenHualiang Jiang
Aug 28, 2009·Trends in Cell Biology·María Yáñez-MóFrancisco Sánchez-Madrid
Dec 25, 2008·Molecular Reproduction and Development·Zhi-Yong HePaul Primakoff
Jan 29, 2013·Cellular Microbiology·Julie PotelLaurence Cocquerel
May 12, 2009·The Biochemical Journal·Stéphanie CharrinEric Rubinstein
Jul 24, 2007·Molecular & Cellular Proteomics : MCP·Oleg V KovalenkoMartin E Hemler
Apr 15, 2017·Biochemical Society Transactions·Ibrahim H YaseenLynda J Partridge
Jun 18, 2017·Biochemical Society Transactions·Sjoerd J van DeventerAnnemiek B van Spriel
Aug 25, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Alba ZuidemaArnoud Sonnenberg
Nov 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cécile ArduiseFrançois Le Naour
Mar 27, 2018·Asia-Pacific Journal of Clinical Oncology·Rama Rao MallaAnil Kumar Badana
Mar 18, 2020·The FEBS Journal·Viviana NevianiPiet Gros
Feb 20, 2021·Experimental Biology and Medicine·Aurelio LoricoGiuseppe Pizzorno
May 22, 2020·The Journal of Physical Chemistry. B·Ivan Castello-SerranoIlya Levental
Sep 16, 2021·The Biochemical Journal·Daniel WirthKalina Hristova
Dec 18, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lun LiHongying Zhong

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