PMID: 9171083Jun 15, 1997Paper

Contrasting effects of single stranded DNA binding protein on the activity of uracil DNA glycosylase from Escherichia coli towards different DNA substrates

Nucleic Acids Research
N V Kumar, U Varshney

Abstract

Excision of uracil from tetraloop hairpins and single stranded ('unstructured') oligodeoxyribonucleotides by Escherichia coli uracil DNA glycosylase has been investigated. We show that, compared with a single stranded reference substrate, uracil from the first, second, third and the fourth positions of the loops is excised with highly variable efficiencies of 3.21, 0.37, 5.9 and 66.8%, respectively. More importantly, inclusion of E.coli single stranded DNA binding protein (SSB) in the reactions resulted in approximately 7-140-fold increase in the efficiency of uracil excision from the first, second or the third position in the loop but showed no significant effect on its excision from the fourth position. In contrast, the presence of SSB decreased uracil excision from the single stranded ('unstructured') substrates approximately 2-3-fold. The kinetic studies show that the increased efficiency of uracil release from the first, second and the third positions of the tetraloops is due to a combination of both the improved substrate binding and a large increase in the catalytic rates. On the other hand, the decreased efficiency of uracil release from the single stranded substrates ('unstructured') is mostly due to the lowering of th...Continue Reading

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Citations

Oct 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Benito BañosMiguel de Vega
Apr 11, 2000·Nucleic Acids Research·M GhoshK V Chary
Oct 22, 2008·Critical Reviews in Biochemistry and Molecular Biology·Robert D SheredaJames L Keck
Apr 16, 2002·European Journal of Biochemistry·Mahua GhoshKandala V R Chary
Jun 5, 1998·The Journal of Biological Chemistry·T BhaduriV Nagaraja
Feb 9, 2006·Chemical Reviews·Mark Lukin, Carlos de Los Santos

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