Contrasting roles of p57(KIP2) and p21(WAF1/CIP1/SDI1) in transplanted human and bovine adrenocortical cells

Experimental Cell Research
M ThomasP J Hornsby

Abstract

Cell transplantation provides a way to compare the regulation of cell proliferation in the same cell type in cell culture and in a vascularized tissue structure in a host animal. The cyclin-dependent kinase inhibitors p57(KIP2), p21(WAF1/CIP1/SDI1) and p27(KIP1) have been extensively studied in cell culture but their role in growth control in tissues is less well understood. In the present experiments we compared the behavior of cell cycle inhibitors in human and bovine adrenocortical cells in culture and following cell transplantation in scid mice. p57 was expressed in the majority of cells in the intact human adrenal cortex. However, double immunofluorescence showed that cells that are in the cell cycle are p57(-) adrenocortical cells, p57 and p27 levels were not affected by inhibition of growth at high cell density, whereas p21 was higher in dividing than growth-inhibited cells. However, p21 was also high in senescent adrenocortical cells. After transplantation of human adrenocortical cells in scid mice, p57 and p27 were observed in most cells in the transplant tissue. Over time the number of p21(+) cells decreased greatly in human adrenocortical cells, but not in bovine adrenocortical cells. This difference correlated with ...Continue Reading

References

Oct 12, 1995·Nature·J BrugarolasG J Hannon
Nov 1, 1995·Experimental Cell Research·L YangP J Hornsby
Oct 1, 1996·Nature Genetics·I HatadaT Mukai
Apr 17, 1997·Nature·W Risau
Oct 9, 1998·Genes & Development·J ZhuJ M Bishop
Jul 1, 1999·Genes & Development·C J Sherr, J M Roberts
Aug 25, 1999·Molecular and Cellular Endocrinology·M Thomas, P J Hornsby
Nov 24, 1999·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·J R TunsteadP J Hornsby
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·D W FelsherJ M Bishop
Dec 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·P J HornsbyL Yang

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Citations

Nov 25, 2006·Mechanisms of Ageing and Development·Qin HuangPeter J Hornsby
Aug 29, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Michael ThomasPeter J Hornsby

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