Contribution of allosteric disulfide in the structural regulation of membrane-bound tissue factor-factor VIIa binary complex

Journal of Biomolecular Structure & Dynamics
Ramesh PrasadProsenjit Sen

Abstract

Two distinct populations, active and cryptic forms of tissue factor (TF), reside on the cell surface. Apart from phospholipid contribution, various models have been introduced to explain decryption/encryption of TF. The proposed model, the switching of Cys186-Cys209 bond of TF, has become the matter of controversy. However, it is well accepted that this disulfide has an immense influence upon ligand factor VIIa (FVIIa) for its binding. However, molecular level understanding for this remains unveiled due to lack of detailed structural information. In this regard, we have performed the molecular dynamic study of membrane-bound TF/TF-FVIIa in both the forms (±Cys186-Cys209 allosteric disulfide bond), individually. Dynamic study depicts that disulfide bond provides structural rigidity of TF in both free and ligand-bound forms. This disulfide bond also governs the conformation of FVIIa structure as well as the binding affinity of FVIIa toward TF. Significant differences in lipid-protein interaction profiles of both the forms of TF in the complex were observed. Two forms of TF, oxidized and reduced, have different structural conformation and behave differentially toward its ligand FVIIa. This disulfide bond not only alters the confor...Continue Reading

References

Oct 29, 1991·Biochemistry·E W DavieW Kisiel
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R Bach, D B Rifkin
Jan 1, 1995·Clinical Immunology and Immunopathology·P E Bigazzi, N R Rose
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Nov 7, 1998·Biochimica Et Biophysica Acta·R F ZwaalE M Bevers
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·A C PikeE Persson
Oct 4, 2000·Physical Review Letters·M VogelT Salditt
Feb 13, 2001·The Journal of Pathology·B A LwaleedA J Cooper
Jun 25, 2002·Journal of Molecular Biology·Raphael GueroisLuis Serrano
Apr 26, 2003·Trends in Biochemical Sciences·Philip J Hogg
Jul 2, 2003·Circulation·Jing ZhangGeorge J Broze
Aug 19, 2003·Nature Structural Biology·Mingdong HuangBarbara C Furie
May 1, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Nigel Mackman
May 21, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Mattias BeltingWolfram Ruf
Oct 14, 2005·Journal of Computational Chemistry·James C PhillipsKlaus Schulten
Jan 1, 1968·The Journal of Clinical Investigation·Y Nemerson
Jun 14, 2006·Biochemistry·Bryan SchmidtPhilip J Hogg
Sep 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jasimuddin AhamedWolfram Ruf
Sep 28, 2006·Biochemistry·Vivien M ChenPhilip J Hogg
Sep 28, 2006·Journal of Thrombosis and Haemostasis : JTH·V M Chen, P J Hogg
Jan 4, 2007·The Journal of Biological Chemistry·Andrew W ShawJames H Morrissey
Jul 14, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Saulius ButenasKenneth G Mann
Mar 9, 2012·Thrombosis Research·L Vijaya Mohan RaoUsha R Pendurthi
Dec 12, 2012·The Journal of Biological Chemistry·Pei WangLiangwei Zhong
Feb 26, 2013·Biochimica Et Biophysica Acta·Jolanta Krudysz-AmbloSaulius Butenas
Apr 30, 2013·Journal of Thrombosis and Haemostasis : JTH·H KothariL V M Rao
Apr 30, 1998·The Journal of Physical Chemistry. B·A D MacKerellM Karplus
Jul 16, 2014·Journal of Theoretical & Computational Chemistry·Lin LiEmil Alexov
Nov 19, 2014·Journal of Thrombosis and Haemostasis : JTH·J J MadsenO H Olsen
Apr 15, 2016·International Journal of Molecular Sciences·Marharyta PetukhEmil Alexov

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Software Mentioned

DelPhi
SASA
NAMD
SAAMBE
CHARMM
Accelrys Discovery Studio
CHARMM builder
FoldX
MEMBPLUGIN
Psfgen

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.