PMID: 9418188Jan 7, 1998Paper

Contribution of antibody to neutrophil-mediated killing of Enterococcus faecalis

Journal of Clinical Immunology
M J GaglaniM S Edwards

Abstract

Late-onset septicemia due to Enterococcus faecalis is common among very low-birth weight neonates. These infants have low concentrations of placentally derived IgG and developmentally low levels of complement. The aim of the present study was to determine the contribution of antibody to in vitro neutrophil-mediated phagocytosis of E. faecalis. Antibody alone, as contained in an adult serum pool heated to inactivate complement, promoted only a modest reduction in the initial bacterial inoculum (50 +/- 12%) for 6 of 10 E. faecalis bacterial strains tested and allowed growth of the other four strains. In the presence of complement, NHS promoted > or = 90% reduction in the initial bacterial inoculum of two representative strains at serum concentrations as low as 0.5%. Hypogammaglobulinemic serum supported similar activity only at concentrations above 5%. Purification of IgG and IgM fractions from NHS revealed that IgM had the higher specific activity to promote phagocytic activity. Absorption to remove specific antibody significantly reduced bactericidal activity by normal human serum, complement-deficient sera, and hypogammaglobulinemic serum. Reconstitution of hypogammaglobulinemic serum with antibody as contained in 1% heated no...Continue Reading

Citations

Mar 19, 2004·The Journal of Biological Chemistry·Mattias Collin, Vincent A Fischetti
Sep 30, 2008·Journal of Immunoassay & Immunochemistry·Yun Sang ChoPan Dong Ryu
Jan 9, 2018·Journal of Immunoassay & Immunochemistry·Yun Sang ChoPan Dong Ryu
Aug 9, 2005·Clinical and Diagnostic Laboratory Immunology·Markus HufnagelJohannes Huebner
Jul 13, 2000·FEMS Immunology and Medical Microbiology·R M RakitaB E Murray
Jul 13, 2019·Microbiology Spectrum·Elizabeth FioreMichael S Gilmore

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