PMID: 2124313Jan 1, 1990Paper

Contribution of arachidonate metabolites to basal and thyrotropin releasing-hormone-stimulated release of prolactin from purified lactotrophs in primary culture

Life Sciences
M P JunierJ D Vincent

Abstract

Among the different biochemical pathways which have been suggested to play a role in the control of prolactin (Prl) release from anterior pituitaries, arachidonate and its metabolites have been proposed to be involved in the process of Prl release. In this study we investigated the contribution of arachidonate metabolites to both basal and TRH-stimulated Prl release from perifused lactotrophs in culture (derived from pituitary glands of lactating female rats), which exhibit a high sustained release of Prl in absence of inhibitory input. Inhibition of the general oxidative metabolism of arachidonate by 10(-5) M ETYA or of the arachidonate lipoxygenase metabolism by 10(-5) M NDGA decreased basal Prl release to 45 +/- 10% (n = 3) and 36 +/- 4% (n = 6) of the control release, respectively. Indomethacin, an inhibitor of the cyclooxygenase pathway, was without effect. Of the lipoxygenase metabolites tested at 10(-6) M only 15-HPETE and 15-HETE induced Prl release. 15-HETE elicited prolactin release in a concentration dependent manner with a maximal effect at 10(-6) M (10.72 +/- 3 ng/ml vs control 5.1 +/- 0.8 ng/ml, n = 3). The quantity of Prl release induced by TRH was markedly decreased in the presence of NDGA. However, the fraction...Continue Reading

References

Dec 1, 1978·Molecular and Cellular Endocrinology·G D BarnesR P Ekins
Nov 25, 1976·Nature·J DrenthJ C Vessies
Jan 1, 1986·Annual Review of Biochemistry·P NeedlemanJ B Lefkowith
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P OnaliE Costa
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G D SnyderJ R Falck
Feb 11, 1982·Biochemical and Biophysical Research Communications·S PiloteP Borgeat

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Citations

Jul 24, 2010·Endocrine Reviews·Stanko S StojilkovicRichard Bertram

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