Contribution of ATPase copper transporters in animal but not plant virulence of the crossover pathogen Aspergillus flavus

Virulence
Kunlong YangNancy P Keller

Abstract

The ubiquitous fungus Aspergillus flavus is notorious for contaminating many important crops and food-stuffs with the carcinogenic mycotoxin, aflatoxin. This fungus is also the second most frequent Aspergillus pathogen after A. fumigatus infecting immunosuppressed patients. In many human fungal pathogens including A. fumigatus, the ability to defend from toxic levels of copper (Cu) is essential in pathogenesis. In A. fumigatus, the Cu-fist DNA binding protein, AceA, and the Cu ATPase transporter, CrpA, play critical roles in Cu defense. Here, we show that A. flavus tolerates higher concentrations of Cu than A. fumigatus and other Aspergillus spp. associated with the presence of two homologs of A. fumigatus CrpA termed CrpA and CrpB. Both crpA and crpB are transcriptionally induced by increasing Cu concentrations via AceA activity. Deletion of crpA or crpB alone did not alter high Cu tolerance, suggesting they are redundant. Deletion of both genes resulted in extreme Cu sensitivity that was greater than that following deletion of the regulatory transcription factor aceA. The ΔcrpAΔcrpB and ΔaceA strains were also sensitive to ROI stress. Compared to wild type, these mutants were impaired in the ability to colonize maize seed tre...Continue Reading

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Citations

May 17, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Martzel Antsotegi-UskolaUnai Ugalde
Aug 31, 2020·Virulence·Damaris Lorenzo-GutiérrezLoida López-Fernández
Jan 11, 2020·Frontiers in Microbiology·Su QuJun Tian
Apr 30, 2019·Frontiers in Microbiology·Jinxing SongJihong Jiang
Apr 26, 2019·International Journal of Molecular Sciences·Nicholas RaffaNancy P Keller
Jul 18, 2020·Frontiers in Microbiology·Xin LiuJianrong Shi

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Methods Mentioned

BETA
PCR

Software Mentioned

BLASTP
Fasttree
GraphPad Prism
MAFFT

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Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.