Contribution of calpain activation to early stages of hippocampal damage during oxygen-glucose deprivation.

Brain Research
Michael GrammerJanusz Lipski

Abstract

Calpains are Ca(2+)-activated enzymes which cleave cytoskeletal and other proteins, contributing to neuronal damage in conditions of pathological intracellular Ca(2+) elevation, including stroke. However, the consequences of calpain overactivation have typically been observed hours after insult. To identify the earliest events attributable to calpain activation, and thus potentially isolate calpain substrates involved in acute neuronal damage, we dynamically recorded the effects of calpain inhibition in an in vitro model of stroke. Extracellular DC potentials and fEPSPs were monitored together with changes of light transmittance (as a measure of cell and mitochondrial swelling) and Rh 123 fluorescence (to monitor mitochondrial membrane potential; DeltaPsi(m)) in hippocampal slices obtained from P12-P17 rats. No differences were observed in the latencies of fEPSP disruption or onset of extracellular DC shifts associated with hypoxic spreading depression (HSD) evoked by oxygen-glucose deprivation (OGD) under control conditions or in the presence of calpain inhibitor III (MDL 28170). However, a significant difference was observed in transmitted light signals during OGD with calpain inhibition. Given the potential contribution of m...Continue Reading

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Citations

Jun 26, 2013·Brain Research·Rashika N Karunasinghe, Janusz Lipski
Apr 9, 2011·Progress in Neurobiology·Joao P Lopes, Paula Agostinho
Jul 16, 2014·Biochimica Et Biophysica Acta·Roberta De TullioEdon Melloni

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