Contribution of cell culture additives to the two-dimensional protein patterns of mouse macrophages

Electrophoresis
Ingrid MillerManfred Gemeiner

Abstract

Low levels of fetal calf serum (FCS), used as protein supplement in cell culture medium, were traced in preparations of primary murine macrophages (bone-marrow-derived macrophages (BMM) and peritoneal macrophages (PM)). Main components of this common additive were mapped in 2-DE by means of differential image gel electrophoresis and immunoblotting. Additional washing steps in cell preparation helped to decrease the levels of the four highest abundance foetal serum proteins (serum albumin (SA), alpha1-fetoprotein (AFP), alpha1-antitrypsin (alpha1AT) and transferrin (Tf)) to less than 1% of total protein. Macrophage spot pattern was recorded in parallel and showed little variation. Results presented are supposed to be of general interest for cell preparations with similar background.

Citations

Sep 25, 2010·Parasitology Research·Gloria HejdukAnja Joachim
Sep 23, 2006·Proteomics·Ingrid MillerElisabetta Gianazza
Apr 5, 2011·Proteomics·Ingrid MillerFriedrich Lackner
Jun 4, 2014·Electrophoresis·Linda Joanne RogersDavid Robert McKenzie
Feb 1, 2012·Thrombosis Research·Jolanta M Siller-MatulaBernd Jilma
Mar 31, 2009·Biochimica Et Biophysica Acta·Andrey V KozlovHeinz Redl
May 16, 2014·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·I MillerA C Gutleb

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