May 31, 2020

Contribution of Complex I NADH dehydrogenase to respiratory energy coupling in glucose-grown cultures of Ogataea parapolymorpha

Applied and Environmental Microbiology
Hannes JuergensRobert Mans

Abstract

The thermotolerant yeast Ogataea parapolymorpha (formerly Hansenula polymorpha) is an industrially relevant production host that exhibits a fully respiratory sugar metabolism in aerobic batch cultures. NADH-derived electrons can enter its mitochondrial respiratory chain either via a proton-translocating Complex I NADH-dehydrogenase or via three putative alternative NADH dehydrogenases. This respiratory entry point affects the amount of ATP produced per NADH/O2 consumed and therefore impacts the maximum yield of biomass and/or cellular products from a given amount of substrate. To investigate the physiological importance of Complex I, a wild-type O. parapolymorpha strain and a congenic Complex I-deficient mutant were grown on glucose in aerobic batch, chemostat and retentostat cultures in bioreactors. In batch cultures, both strains exhibited a fully respiratory metabolism and showed the same growth rate and biomass yield, indicating that, under these conditions, the contribution of NADH oxidation via Complex I was negligible. Both strains also exhibited a respiratory metabolism in glucose-limited chemostat cultures, but the Complex I-deficient mutant showed considerably reduced biomass yields on substrate and oxygen, consistent...Continue Reading

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Complex (molecular entity)
Bioreactors
Analysis
Proton-Translocating ATPases
Amino Sugar Metabolic Process
Energy Metabolism
Oxygen Measurement
Oxygen
Volumetric
Patterns

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.

Bioreactors (ASM)

Bioreactors are important devices or systems that utilize living cells or enzymes for a chemical process. These devices carefully control and monitor the environment factors such as pH, nutrients level, and temperature, which can affect the yield in a given process. Discover the latest research here.