May 31, 2020

Contribution of Complex I NADH dehydrogenase to respiratory energy coupling in glucose-grown cultures of Ogataea parapolymorpha

Applied and Environmental Microbiology
Hannes JuergensRobert Mans


The thermotolerant yeast Ogataea parapolymorpha (formerly Hansenula polymorpha) is an industrially relevant production host that exhibits a fully respiratory sugar metabolism in aerobic batch cultures. NADH-derived electrons can enter its mitochondrial respiratory chain either via a proton-translocating Complex I NADH-dehydrogenase or via three putative alternative NADH dehydrogenases. This respiratory entry point affects the amount of ATP produced per NADH/O2 consumed and therefore impacts the maximum yield of biomass and/or cellular products from a given amount of substrate. To investigate the physiological importance of Complex I, a wild-type O. parapolymorpha strain and a congenic Complex I-deficient mutant were grown on glucose in aerobic batch, chemostat and retentostat cultures in bioreactors. In batch cultures, both strains exhibited a fully respiratory metabolism and showed the same growth rate and biomass yield, indicating that, under these conditions, the contribution of NADH oxidation via Complex I was negligible. Both strains also exhibited a respiratory metabolism in glucose-limited chemostat cultures, but the Complex I-deficient mutant showed considerably reduced biomass yields on substrate and oxygen, consistent...Continue Reading

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Mentioned in this Paper

Complex (molecular entity)
Proton-Translocating ATPases
Amino Sugar Metabolic Process
Energy Metabolism
Oxygen Measurement

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