Contribution of cysteine residue to the properties of Listeria monocytogenes listeriolysin O

Canadian Journal of Microbiology
Radosław StachowiakJacek Bielecki

Abstract

Listeriolysin (LLO) is the key virulence factor critical for Listeria monocytogenes pathogenesis. Listerial cytolysin belongs to the family of cholesterol-dependent cytolysins (CDCs), a group of pore-forming toxins produced by related gram-positive bacteria. Most CDCs contain a cysteine residue in the conserved undecapeptide - a sequence that is highly preserved among this group of proteins. Substitutions of cysteine do not always lead to loss of hemolytic activity, questioning the purpose of such strong conservation of this amino acid in the sequence of CDC. The properties of 3 L. monocytogenes strains, a wild type and 2 mutants expressing modified LLO within the cysteine residue, were analyzed in this work. The first of these mutants producing a toxin with cysteine to alanine substitution showed similar features to the wild type except that a thiol-reducing agent was not necessary for hemolytic activity. Another strain secreting LLO containing serine instead of cysteine exhibited strikingly different properties than the wild type. Modified toxin is independent of the reducing reagents, less stable, and shows accelerated kinetics of cytolysis in comparison with the unchanged protein. However, both mutant strains are less invas...Continue Reading

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Citations

Oct 24, 2012·Journal of Biomedicine & Biotechnology·R StachowiakJ Kawiak
Feb 19, 2013·Journal of Biotechnology·Zachary F WallsKyung-Dall Lee
Jul 1, 2016·Future Microbiology·Tobias TenenbaumChristian Schwerk
Sep 3, 2016·G3 : Genes - Genomes - Genetics·Paula McCourtJoseph T Nickels
Aug 25, 2020·3 Biotech·Deepak KalaAnkur Kaushal

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