Contribution of disulfide bonds and calcium to Molluscan hemocyanin stability

Zeitschrift Für Naturforschung. C, a Journal of Biosciences
D N GeorgievaC Betzel

Abstract

Disulfide bonds and calcium ions contribute significantly to the stability of the hemocyanin from the mollusc Rapana thomasiana grosse (gastropod). An extremely powerful protective effect of Ca2+ at a concentration of 100 mM (100% protection) against the destructive effect of reductants like dithiothreitol was observed. This is important for the practical application of molluscan hemocyanins in experimental biochemistry, immunology and medicine. The reduction of the disulfide bonds in the Rapana hemocyanin leads to a 20% decrease of the a-helical structure. The S-S bonds contribute significantly to the free energy of stabilization in water increasing delta G(D)H2O by 6.9 kJ mol (-1) The data are related to the X-ray model of the Rapana hemocyanin functional unit RtH2e. The results of this study can be of common validity for related respiratory proteins because the cysteine residues are conserved in all sequences of molluscan hemocyanins published so far.

Citations

Nov 28, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Negar Talaei ZanjaniFariba Dehghani
Mar 27, 2012·Biochimica Et Biophysica Acta·Krassimira IdakievaConstant Gielens
Jan 14, 2021·Journal of Molecular Evolution·Gabriela Giannina SchäferBernhard Lieb

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