Contribution of DNA methylation and EZH2 in SRBC down-regulation in gastric cancer.

Molecular Biology Reports
Shiva RezaeiReza Safaralizadeh

Abstract

Gastric cancer (GC), a high mortality malignancy, is induced by genetic and epigenetic factors. DNA and histone methylation play critical roles in tumor suppressor genes inactivation. SRBC (serum deprivation response factor-related gene product that binds to the c-kinase), suggested as a tumor suppressor gene, participates in apoptosis, tumor chemoresistance and DNA damage response and is repressed in various cancers. Inspecting the mechanisms underlying SRBC suppression is important for cancer treatments. We investigated SRBC promoter DNA methylation status and expression of SRBC and EZH2 histone methyltrasferase in gastric cancer. Also, we surveyed SRBC expression after 5-azacitidine and UNC1999 treatments of AGS cell line. In current work, we used gastric adenocarcinoma tissues, marginal samples and normal gastric biopsies. DNA methylation was detected by Methylation- Specific PCR and mRNA expression was measured by Real-Time PCR. SRBC promoter methylation analysis, showed fully and partial methylated versions that were associated with patient's age (p = 0.001). SRBC expression significantly decreased in GC compare with marginal and normal samples (p-value < 0.001). EZH2 showed remarkable up-regulation in GC than controls an...Continue Reading

References

Sep 26, 2007·Human Molecular Genetics·Manel Esteller
Dec 7, 2007·International Journal of Cancer. Journal International Du Cancer·Jin-Hee LeeSung-Gil Chi
Apr 24, 2008·The Journal of Biological Chemistry·Satoshi FujiiAtsushi Ochiai
Aug 28, 2009·Seminars in Reproductive Medicine·Cathérine DupontCarol A Brenner
Apr 29, 2010·Acta Obstetricia Et Gynecologica Scandinavica·Seo-Yun TongSeon-Kyung Lee
Oct 8, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jin-Hee LeeSung-Gil Chi
Jul 12, 2012·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Lisa D MooreGuoping Fan
Sep 22, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·Long-Jun HeDan Xie
Nov 26, 2013·Journal of the National Cancer Institute·Catia MoutinhoManel Esteller
Jan 28, 2014·Genomics & Informatics·Jae Duk Choi, Jong-Soo Lee
Sep 23, 2014·Biochimie·Reshu GuptaBorhane Annabi
May 4, 2016·Cold Spring Harbor Perspectives in Biology·Geneviève Almouzni, Howard Cedar
Nov 28, 2017·Journal of Gastrointestinal Cancer·Reza SafaralizadehSeyed-Yaghob Moaddab
Feb 13, 2018·Oncology Letters·Zhuanpeng ChenJie Cao
Aug 29, 2018·Journal of Gastrointestinal Oncology·Seyedeh Saideh DaryabariBehrooz Shokouhi
Dec 14, 2018·Molecular Diagnosis & Therapy·Katarzyna KamińskaMarzena Anna Lewandowska
Jan 4, 2019·Journal of Gastrointestinal Oncology·Arash Azimzadeh-IsfanjaniSeyyed-Yaghoub Moaddab

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
surgical resection
PCR

Software Mentioned

Quantity One
SPSS

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis