Contribution of ebola virus glycoprotein, nucleoprotein, and VP24 to budding of VP40 virus-like particles

Journal of Virology
Jillian M LicataRonald N Harty

Abstract

The VP40 matrix protein of Ebola virus buds from cells in the form of virus-like particles (VLPs) and plays a central role in virus assembly and budding. In this study, we utilized a functional budding assay and cotransfection experiments to examine the contributions of the glycoprotein (GP), nucleoprotein (NP), and VP24 of Ebola virus in facilitating release of VP40 VLPs. We demonstrate that VP24 alone does not affect VP40 VLP release, whereas NP and GP enhance release of VP40 VLPs, individually and to a greater degree in concert. We demonstrate further the following: (i). VP40 L domains are not required for GP-mediated enhancement of budding; (ii). the membrane-bound form of GP is necessary for enhancement of VP40 VLP release; (iii). NP appears to physically interact with VP40 as judged by detection of NP in VP40-containing VLPs; and (iv). the C-terminal 50 amino acids of NP may be important for interacting with and enhancing release of VP40 VLPs. These findings provide a more complete understanding of the role of VP40 and additional Ebola virus proteins during budding.

References

Jan 1, 1996·Advances in Virus Research·H Feldmann, H D Klenk
May 20, 1998·The Journal of the Association of Nurses in AIDS Care : JANAC·H M Miramontes
Jun 6, 1998·Virology·V E VolchkovH Feldmann
Jan 16, 1999·The Journal of Biological Chemistry·P ScheiffeleK Simons
Nov 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·R N HartyF P Hayes
Dec 2, 2000·Nature Medicine·H D Klenk
Apr 21, 2001·Virology·J TimminsW Weissenhorn
May 3, 2001·Journal of Virology·L D JasenoskyY Kawaoka
Oct 13, 2001·Virus Research·H D Klenk, H Feldmann
Mar 6, 2002·The Journal of Experimental Medicine·Sina BavariM Javad Aman
Mar 22, 2002·Journal of Virology·Anthony P SchmittRobert A Lamb
Apr 23, 2002·Journal of Virology·Eric O Freed
Apr 23, 2002·Journal of Virology·Takeshi NodaYoshihiro Kawaoka
Feb 1, 2003·Journal of Molecular Biology·Joanna TimminsWinfried Weissenhorn
Mar 26, 2003·The Journal of General Virology·John A G BriggsStephen D Fuller

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Citations

Aug 24, 2006·Virus Genes·Ziying HanRonald N Harty
Feb 7, 2013·Virologica Sinica·Karen A O MartinsSina Bavari
Jan 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Peter HalfmannYoshihiro Kawaoka
Jan 31, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rachel L KaletskyPaul Bates
Sep 8, 2010·Proceedings of the National Academy of Sciences of the United States of America·Derek DubeJudith M White
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Tanmay A M BharatJohn A G Briggs
Dec 6, 2007·The Journal of Infectious Diseases·Kelly L WarfieldSina Bavari
Dec 6, 2007·The Journal of Infectious Diseases·Takeshi NodaYoshihiro Kawaoka
Dec 6, 2007·The Journal of Infectious Diseases·Kelly L WarfieldSina Bavari
Oct 19, 2011·The Journal of Infectious Diseases·Yuliang LiuRonald N Harty
Nov 15, 2011·The Journal of Infectious Diseases·Annika KühlStefan Pöhlmann
Oct 19, 2011·The Journal of Infectious Diseases·Ayaka IwasaYoshihiro Kawaoka
Oct 19, 2011·The Journal of Infectious Diseases·Valentina A VolchkovaViktor E Volchkov
Oct 19, 2011·The Journal of Infectious Diseases·Reed S ShabmanChristopher F Basler
Oct 19, 2011·The Journal of Infectious Diseases·Kelly L Warfield, M Javad Aman
Oct 19, 2011·The Journal of Infectious Diseases·Annika KühlStefan Pöhlmann
Mar 2, 2012·Science Translational Medicine·Mayra GarcíaGary J Nabel
Sep 24, 2010·Clinical and Vaccine Immunology : CVI·Eri NakayamaAyato Takada
Aug 6, 2010·Journal of Virology·Sheli R RadoshitzkySina Bavari
Aug 19, 2007·Journal of Virology·Sarah E McCarthyRonald N Harty
Dec 17, 2010·Journal of Virology·Jesica M Levingston MacleodNora Lopez
Jan 19, 2007·Journal of Virology·Takeshi NodaYoshihiro Kawaoka
Jan 15, 2010·Journal of Virology·Katrin SchlieWolfgang Garten
May 16, 2006·Journal of Virology·St Patrick ReidChristopher F Basler
Jan 29, 2010·Journal of Virology·Allison GrosethStephan Becker
Jul 30, 2005·Journal of Virology·Gabriele NeumannYoshihiro Kawaoka
Oct 18, 2005·Journal of Virology·Sandra BambergStephan Becker
Jan 15, 2005·Journal of Virology·Thomas HoenenWinfried Weissenhorn
Mar 31, 2006·Journal of Virology·Shinji WatanabeYoshihiro Kawaoka
Feb 9, 2012·Advances in Virology·Shuzo Urata, Juan Carlos de la Torre
Dec 22, 2005·Virology Journal·Ziying Han, Ronald N Harty

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