Abstract
We investigated the biological significance of estrogen receptors (ER) in NIH3T3 cell transformation by the [12Val] K-Ras mutant. This mutant enhanced the steady state level of ER. Cells expressing mutant K-Ras (K12V cell) were tumorigenic. To determine the role of ER accumulation in Ras-transformed cells, we developed cells (KwtER cells) that overexpressed both wild-type (wt) K-Ras and ER, and found these cells were also tumorigenic. E2 stimulated the transcriptional activity by ER dominantly in K12V cells. However, only partial activation of ER by E2 was seen in KwtER cells. In the presence of 10% serum in media, the activation of ER appeared only in transformed KtwER and K12V cells, suggesting that two independently transmitted signals, the E2-ER binding and the ER-AF1 activation, are necessary for ER activation and that the dominant activation of ER might be involved in Ras-mediated cell transformation. Co-expression of progesterone receptor (PR) with mutant K-Ras led to suppression of tumorigenicity and inhibition of the activation of ER. The antisense oligomers complementary to the ER suppressed proliferation and transformed phenotypes of K12V cells. These observations support the importance of ER in Ras-mediated cell tra...Continue Reading
Citations
Nov 25, 2000·Breast Cancer : the Journal of the Japanese Breast Cancer Society·K KatoN Wake
Apr 9, 2005·Experimental Cell Research·Shinji HoriuchiNorio Wake
May 27, 2008·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Julia TurbinerJosé Palacios
Dec 30, 2009·Oligonucleotides·Maiko HiguchiAkira Murakami
Apr 30, 2013·Oncology Reports·Tomoko YonedaNorio Wake
Apr 2, 2013·Oncogene·P RajbhandariE T Alarid
Apr 12, 2014·BMC Gastroenterology·Carlos Gil FerreiraMariano Zalis
May 6, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yan ZhaoMinghong Zhao
Feb 13, 2009·Gynecologic Oncology·Akira TakahashiNorio Wake
Apr 22, 2004·Clinics in Chest Medicine·M Patricia Rivera, Diane E Stover
Feb 20, 2007·Gynecologic Oncology·Shin SugaNorio Wake
Aug 22, 2015·BMC Medical Genetics·Kanako OkamotoKiyoko Kato
Apr 25, 2000·The Tohoku Journal of Experimental Medicine·K TanoguchiA Yajima
Mar 21, 2002·Clinics in Chest Medicine·Lynn T Tanoue, Ronald B Ponn
Jan 5, 2002·Physiological Genomics·I KuhnP H Johnson
Jan 10, 2002·The Journal of Biological Chemistry·Kiyoko KatoNorio Wake
May 28, 2015·Oncotarget·Kyoung-Hwa KooKang-Yell Choi
Nov 5, 2021·PloS One·Morgan T WalcheckSean M Ronnekleiv-Kelly