Contribution of extracellular ice formation and the solution effects to the freezing injury of PC-3 cells suspended in NaCl solutions

Cryobiology
Hiroshi Takamatsu, Sylwia Zawlodzka

Abstract

The mechanism of cell injury during slow freezing was examined using PC-3 human prostate adenocarcinoma cells suspended in NaCl solutions. The objective was to evaluate contribution of extracellular ice and the 'solution effects' to freezing injury separately. The solution effects that designate the influence of elevated concentration were evaluated from a pseudo-freezing experiment, where cells were subjected to the milieu that simulated a freeze-thaw process by changing the NaCl concentration and the temperature at the same time. The effect of extracellular ice formation on cell injury was then estimated from the difference in cell survival between the pseudo-freezing experiment and a corresponding freezing experiment. When cells were frozen to a relatively higher freezing temperature at -10 degrees C, about 30% of cells were damaged mostly due to extracellular ice formation, because the concentration increase without ice formation to 2.5-M NaCl, i.e., the equilibrium concentration at -10 degrees C, had no effect on cell survival. In contrast, in the case of the lower freezing temperature at -20 degrees C, about 90% of cells were injured by both effects, particularly 60-80% by the solution effects among them. The present resu...Continue Reading

References

Jun 1, 1973·Cryobiology·A DawG J Morris
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·W J Gordon-Kamm, P L Steponkus
Feb 25, 2003·Journal of Biomechanical Engineering·Hiroshi Takamatsu, Norihiko Kumagae
Nov 3, 2004·Journal of Biomechanics·Hiroshi TakamatsuHideki Sumimoto
Nov 17, 2004·Journal of Biomechanical Engineering·Hiroshi TakamatsuMotoo Fujii
Feb 16, 2005·Cryobiology·Sylwia Zawlodzka, Hiroshi Takamatsu

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Citations

Nov 9, 2010·Cryobiology·Vishard RagoonananAlptekin Aksan
Oct 16, 2012·Theriogenology·J D BensonJ K Critser
Feb 26, 2014·Critical Reviews in Food Science and Nutrition·Shuna ZhaoSang-Moo Kim
Nov 20, 2020·Cardiovascular and Interventional Radiology·Masanori YamashitaKei Yamada
Aug 30, 2019·Mathematical Biosciences·Daniel M AndersonAnthony J Kearsley
Mar 26, 2021·BMC Biology·Sanja BojicJoão Pedro de Magalhães
Apr 14, 2021·BMJ : British Medical Journal·Shiva P PonamgiPeter A Noseworthy

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