Contribution of Lactococcus lactis cell envelope proteinase specificity to peptide accumulation and bitterness in reduced-fat Cheddar cheese.

Applied and Environmental Microbiology
Jeffery R BroadbentJames L Steele

Abstract

Bitterness is a flavor defect in Cheddar cheese that limits consumer acceptance, and specificity of the Lactococcus lactis extracellular proteinase (lactocepin) is widely believed to be a key factor in the development of bitter cheese. To better define the contribution of this enzyme to bitterness, we investigated peptide accumulation and bitterness in 50% reduced-fat Cheddar cheese manufactured with single isogenic strains of Lactococcus lactis as the only starter. Four isogens were developed for the study; one was lactocepin negative, and the others produced a lactocepin with group a, e, or h specificity. Analysis of cheese aqueous extracts by reversed-phase high-pressure liquid chromatography confirmed that accumulation of alpha(S1)-casein (f 1-23)-derived peptides f 1-9, f 1-13, f 1-16, and f 1-17 in cheese was directly influenced by lactocepin specificity. Trained sensory panelists demonstrated that Cheddar cheese made with isogenic starters that produced group a, e, or h lactocepin was significantly more bitter than cheese made with a proteinase-negative isogen and that propensity for bitterness was highest in cells that produced group h lactocepin. These results confirm the role of starter proteinase in bitterness and su...Continue Reading

References

Jan 1, 1988·Applied and Environmental Microbiology·J KokG Venema
Jun 1, 1972·Applied Microbiology·L L McKayE A Zottola
Sep 1, 1983·Applied and Environmental Microbiology·D G Anderson, L L McKay
Oct 1, 1996·Antonie van Leeuwenhoek·E R KunjiW N Konings
Jun 3, 1998·Applied and Environmental Microbiology·D LowJ R Broadbent
Nov 13, 1998·Journal of Bacteriology·G BuistJ Kok
Dec 1, 2000·Applied and Environmental Microbiology·B Flambard, V Juillard
Mar 1, 1996·Applied and Environmental Microbiology·S L CaldwellJ R Broadbent
Sep 1, 1922·Journal of Bacteriology·J H Brown, P E Howe

❮ Previous
Next ❯

Citations

Apr 22, 2006·Applied Microbiology and Biotechnology·Kirsi SavijokiPekka Varmanen
Jul 25, 2003·Journal of Chromatography. a·M Careri, A Mangia
Feb 7, 2003·Applied and Environmental Microbiology·Yo-Shen ChenJames L Steele
Nov 21, 2007·Trends in Microbiology·Erika A Pfeiler, Todd R Klaenhammer
May 16, 2006·Journal of Applied Microbiology·J R BroadbentJ L Steele
May 4, 2011·Journal of Food Science·Ana R MadureiraF Xavier Malcata
Jan 23, 2016·Dairy Science & Technology·Philip KelleherDouwe van Sinderen
Jan 22, 2013·Gut Microbes·Gabriele HörmannspergerDirk Haller
Dec 30, 2014·Applied Biochemistry and Biotechnology·Monika GarbowskaAnna Berthold-Pluta
Jul 21, 2004·Journal of Dairy Science·MaryAnne Drake
Mar 22, 2005·Journal of Dairy Science·V FallicoG Licitra
Jun 24, 2020·FEMS Microbiology Letters·José María Coll-MarquésGaspar Pérez-Martínez
May 12, 2020·Journal of Dairy Science·Hui FanYongfu Chen
Apr 26, 2019·Frontiers in Microbiology·Philip KelleherDouwe van Sinderen
Oct 1, 2003·Comprehensive Reviews in Food Science and Food Safety·T K SinghK R Cadwallader
Jul 31, 2014·Journal of Agricultural and Food Chemistry·Konstantinia KarametsiDevin G Peterson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.