Contribution of light chain rearrangement in peripheral B cells to the generation of high-affinity antibodies

European Journal of Immunology
Masaki MagariHitoshi Ohmori

Abstract

Recently, peripheral B cells have been shown to undergo secondary V(D)J rearrangement of immunoglobulin genes, but the physiological role of this event has not been fully elucidated. To investigate whether rearrangement of L chain genes in the periphery is involved in the generation of high-affinity antibodies (Ab), we used the 17.2.25 rearranged VHDJH gene (VHT)-knockin mouse whose B cell diversity is limited due to the expression of the site-directed transgene. Immunization of the mouse with p-nitrophenylacetyl (pNP)-conjugated chicken gamma-globulin preferentially led to the production of anti-pNP IgG Ab comprised of non-VHT-encoded H chains and lambda chains. lambda(+) IgG constituted a majority of high-affinity Ab to this hapten. RAG-2 mRNA and the recombination signal sequence break of the lambda1 gene increased in the draining lymph node of immunized mice, but not of nonimmunized animals. There was a close correlation between the levels of these parameters implicating lambda gene rearrangement and the production of lambda(+ )high-affinity anti-pNP IgG. These observations were reproduced in RAG-1-deficient mice that were reconstituted with the spleen cells ofthe knockin mouse. Thus, our findings suggest that L chain rearr...Continue Reading

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Citations

Jul 21, 2010·Molecular Biotechnology·Jeffrey S SwersK Dane Wittrup
Mar 14, 2003·Current Opinion in Immunology·David Nemazee, Kristin A Hogquist
Apr 20, 2006·Molecular Medicine·Chrysoula BelessiNikolaos Laoutaris
Oct 21, 2004·European Journal of Immunology·Lena ErlandssonInga-Lill Mårtensson
Jul 15, 2005·Annals of the New York Academy of Sciences·Sophie HillionChristophe Jamin
Dec 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Naoki KanayamaHitoshi Ohmori
Jan 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Naoki KanayamaHitoshi Ohmori
Aug 22, 2006·Scandinavian Journal of Immunology·S HillionC Jamin
Nov 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sophie HillionChristophe Jamin
Nov 26, 2015·Immunology Letters·Hyeon-Ju KangSang-Hoon Cha

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