Contribution of microdissection for the detection of microsatellite instability in colorectal cancer

Human Pathology
Marie DanjouxJanick Selves

Abstract

The determination ofmicrosatellite instability (MSI) is an important step in the identification of familial colorectal cancer such as hereditary nonpolyposis colon cancer. It could also be of interest in the therapeutic management of sporadic cancer. International criteria for the determination of MSI have been published, recommending the use of microdissection. The aim of this work was to evaluate the impact of contaminant normal DNA in tumor samples for MSI assessment in colorectal cancer using a microdissection technique. We performed a comparative analysis of the microsatellite status between total DNA (DNA extracted from whole tumor samples) and microdissected DNA in 3 different regions from 23 cases of colorectal cancer. Six microsatellites were amplified using fluorescent polymerase chain reaction. We analyzed 9 cases with MSI and 14 cases without instability, with similar results between total DNA and microdissected DNA. Moreover, within a same tumor, the MSI phenotype was observed regardless of the region analyzed. Thus, this work shows the reproducibility of the MSI phenotype throughout a tumor. However, we observed a regional heterogeneity of the MSI profile, consisting of variations in the number and the size of uns...Continue Reading

Citations

May 25, 2010·Pathology Oncology Research : POR·Francois SaucyGian Dorta
Jan 28, 2012·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Florence Boissière-MichotFrédéric Bibeau
Nov 6, 2010·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Ermanno NardonGiorgio Stanta
Apr 7, 2012·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Delphine BonnetRosine Guimbaud

Apr 7, 2020·Annales de pathologie·Frédéric BibeauJanick Selves

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