Contribution of neutrophil elastase to the lysis of obliterative thrombi in the context of their platelet and fibrin content.

Thrombosis Research
Gyöngyi RábaiKrasimir Kolev

Abstract

Leukocytes invade newly formed thrombi through interactions with platelets and fibrin and later contribute to the removal of fibrin deposits mainly through the action of neutrophil elastase. The present study attempts to express in quantitative terms the impact of neutrophils on the lytic processes in obliterative thrombi based on the local presence of elastase-specific fibrin degradation products (NE-FDP) in relation to the leukocyte, platelet and fibrin content of thrombi. Immunofluorescent detection of fibrin, NE-FDP and platelet antigens was performed in sections of thrombi from 28 patients subjected to thrombectomy in combination with DNA-staining for identification of nucleated cells. The digitalized fluorescent microscopic images were decomposed according to the color channel of each thrombus constituent. The integrated intensity values for all thrombus constituents were statistically evaluated with correlation, hierarchical agglomerative clustering , Hotelling'sT(2) and F-statistics. Association between NE-FDP and leukocyte content of thrombi is evidenced by a significant Pearson correlation coefficient of 0.71 (p=0.00002). Cluster analysis reveals two classes of thrombi according to NE-FDP, leukocyte and platelet conte...Continue Reading

References

Apr 1, 1988·The Biochemical Journal·M A SelakJ B Smith
Jan 1, 1984·Seminars in Thrombosis and Hemostasis·L A Moroz
Jun 5, 1980·Biochimica Et Biophysica Acta·E F Plow
Jun 11, 1998·Circulation Research·E KomorowiczR Machovich
Dec 3, 2005·The Journal of Clinical Investigation·Bruce Furie, Barbara C Furie
Jun 29, 2007·Thrombosis and Haemostasis·Zaverio M Ruggeri
May 26, 2009·Nature Medicine·Warwick S NesbittShaun P Jackson

❮ Previous
Next ❯

Citations

Jan 8, 2013·The Journal of Biological Chemistry·Colin LongstaffKrasimir Kolev

❮ Previous
Next ❯

Software Mentioned

Matlab
Statistica

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Canadian Respiratory Journal : Journal of the Canadian Thoracic Society
Lisa FerrignoRiyad Karmy-Jones
Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Guanrao Chen, Yang Dai
Cardiovascular and Interventional Radiology
M A WeinerM Silane
American Journal of Mental Retardation : AJMR
A B SilversteinJ F White
© 2021 Meta ULC. All rights reserved