PMID: 9445401Mar 7, 1998Paper

Contribution of newly synthesized cholesterol to rat plasma and bile determined by mass isotopomer distribution analysis: bile-salt flux promotes secretion of newly synthesized cholesterol into bile

The Biochemical Journal
Robert H J BandsmaF Kuipers

Abstract

To quantify the contribution of newly synthesized cholesterol to total plasma and biliary cholesterol under physiological conditions, unrestrained rats were infused intravenously with [1-13C]acetate (0. 6mmol/h per kg) from 12:00 to 24:00 h, and fractional and absolute cholesterol-synthesis rates were determined by mass isotopomer distribution analysis (MIDA). As bile diversion leads to changes in cholesterol metabolism, rats were equipped with permanent catheters in the bile duct and duodenum, allowing sampling of small amounts of bile from an intact enterohepatic circulation. For comparison, rats with chronic bile diversion were also studied. Fractional synthesis of plasma cholesterol was 10.8+/-1.7% (mean+/-S.D.) after 12 h in rats with intact circulation. Fractional synthesis of biliary cholesterol was significantly higher than that of plasma cholesterol, i.e. 16.5+/-2.0% (P<0.05) after 12 h. In contrast, no differences between fractional synthesis of cholesterol in plasma and bile were found in bile-diverted animals (31.8+/-2.1 and 33.1+/-3.3% respectively after 12 h). The calculated absolute rate of cholesterol biosynthesis increased from 53+/-10 to 221+/-19 micromol/day per kg after bile diversion. A comparison of MIDA r...Continue Reading

Citations

Jul 1, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J ScheibnerE F Stange
May 2, 2015·Trends in Pharmacological Sciences·Ryan E Temel, J Mark Brown
May 28, 2013·Biochimica Et Biophysica Acta·Stephen F PrevisThomas P Roddy
Jul 30, 2005·Mass Spectrometry Reviews·Susumu Ando, Yasukazu Tanaka
Apr 6, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Cédric Le MayPhilippe Costet
May 17, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Gemma BrufauFolkert Kuipers
May 7, 2009·The Journal of Biological Chemistry·Jelske N van der VeenFolkert Kuipers
Jan 21, 2012·Current Opinion in Lipidology·Ryan E Temel, J Mark Brown
Jul 10, 2002·The Journal of Biological Chemistry·Torsten PlōschFolkert Kuipers

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