PMID: 9190807Jun 1, 1997Paper

Contribution of partner switching and SpoIIAA cycling to regulation of sigmaF activity in sporulating Bacillus subtilis

Journal of Bacteriology
T MagninM D Yudkin

Abstract

sigmaF, the first compartment-specific transcription factor in sporulating Bacillus subtilis, is negatively regulated by an anti-sigma factor, SpoIIAB. SpoIIAB has an alternative binding partner, SpoIIAA. To see whether (as has been proposed) SpoIIAB's binding preference for SpoIIAA or sigmaF depends on the nature of the adenine nucleotide present, we used surface plasmon resonance to measure the dissociation constants of the three complexes SpoIIAA-SpoIIAB-ADP, sigmaF-SpoIIAB-ADP, and sigmaF-SpoIIAB-ATP. The results suggested that SpoIIAB's choice of binding partner is unlikely to depend on the ATP/ADP ratio in the cell. The intracellular concentrations of sigmaF, SpoIIAB, SpoIIAA, and SpoIIAA-phosphate (SpoIIAA-P) were measured by quantitative immunoblotting between 0 and 3 h after the beginning of sporulation (t0 to t3). sigmaF and SpoIIAB were barely detectable at t0, but their concentrations increased in parallel to reach maxima at about t1.5. SpoIIAA-P increased steadily to a maximum at t3, but nonphosphorylated SpoIIAA was detectable only from t1.5, reached a maximum at t2.5, and then declined. Kinetic studies of the phosphorylation of SpoIIAA catalyzed by SpoIIAB suggested that the reaction was limited by a very slow re...Continue Reading

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Citations

Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·H KovacsM D Yudkin
Apr 14, 1999·Molecular Microbiology·L KroosY T Yu
Jul 28, 2005·Molecular Microbiology·Imrich Barák, Anthony J Wilkinson
May 20, 2006·Nature·Dagmar IberIain D Campbell
Jan 28, 2004·The Journal of Biological Chemistry·Karen CarniolRichard Losick
Mar 4, 2009·Artificial Life·Dafyd J Jenkins, Dov J Stekel
Feb 3, 2018·Environmental Microbiology Reports·Sophie BouilletChantal Iobbi-Nivol
May 20, 2003·Molecular Microbiology·Simon L DoveAnn Hochschild
Nov 5, 2016·The Journal of Biological Chemistry·Sophie BouilletChantal Iobbi-Nivol
May 10, 2000·The Journal of Biological Chemistry·M A Cervin, G B Spiegelman
Aug 1, 2010·Environmental Microbiology·Andrew R Morris, Karen L Visick
Jan 19, 1999·Annual Review of Microbiology·K T Hughes, K Mathee
Sep 25, 2002·Journal of Bacteriology·Olivier DelumeauMichael D Yudkin
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·David W Hilbert, Patrick J Piggot
Mar 6, 1999·Current Opinion in Microbiology·P A Levin, A D Grossman
May 14, 1999·Current Opinion in Microbiology·J D Helmann

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