Contribution of permeability and sensitivity to inhibition of DNA synthesis in determining susceptibilities of Escherichia coli, Pseudomonas aeruginosa, and Alcaligenes faecalis to ciprofloxacin.

Antimicrobial Agents and Chemotherapy
J BedardL E Bryan

Abstract

To examine the correlation between bacterial cell susceptibility to ciprofloxacin and the magnitude of uptake and cell target sensitivity, the relative contribution of ciprofloxacin accumulation in intact cells and its ability to inhibit DNA synthesis were investigated among strains of Escherichia coli, Pseudomonas aeruginosa, and Alcaligenes faecalis. Uptake studies of [14C]ciprofloxacin demonstrated diffusion kinetics for P. aeruginosa and E. coli. Ciprofloxacin was more readily removed from E. coli J53 and A. faecalis ATCC 19018 by washing than from P. aeruginosa PAO503. These results indicate that the process of cell accumulation is different for P. aeruginosa in that the drug is firmly bound at an extracellular site. Whatever the washing conditions, A. faecalis accumulated less drug than either of the other two bacteria. Magnesium chloride (10 mM) caused a substantial decrease of ciprofloxacin accumulated and an increase in the MIC, depending upon the nature of the medium. The addition of carbonyl cyanide m-chlorophenylhydrazone caused a variable increase in drug accumulated, depending on the medium and the bacterial strain. The concentration of ciprofloxacin required to obtain 50% inhibition (ID50) of DNA synthesis for P....Continue Reading

References

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Citations

Apr 1, 1991·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·D C Hooper, J S Wolfson
Apr 1, 1991·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L E Bryan, J Bedard
Jan 1, 1993·International Journal of Antimicrobial Agents·A Bryskier
Mar 1, 1991·Antimicrobial Agents and Chemotherapy·M Michea-HamzehpourJ C Pechere
Aug 1, 1992·Antimicrobial Agents and Chemotherapy·C McCaffreyN H Georgopapadakou
Apr 1, 1995·Journal of Chemotherapy·M H TeixeiraF Teixeira
Sep 10, 2016·The Journal of Antimicrobial Chemotherapy·Jason G GlanzerGreg G Oakley
Jul 3, 2018·ACS Infectious Diseases·Ramkumar IyerAlice L Erwin

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