Contribution of phospholipase D in endothelin-1-mediated extracellular signal-regulated kinase activation and proliferation in rat uterine leiomyoma cells

Biology of Reproduction
Philippe RobinZahra Tanfin

Abstract

Endothelin (ET)-1 is a mitogenic factor in numerous cell types, including rat myometrial cells. In the present study, we investigated the potential role of ET-1 in the proliferation of tumoral uterine smooth muscle cells (ELT-3 cells). We found that ET-1 exerted a more potent mitogenic effect in ELT-3 cells than in normal myometrial cells, as indicated by the increase in [3H]thymidine incorporation, cell number, and bromodeoxyuridine incorporation. The ET-1 was more efficient than platelet-derived growth factor and epidermal growth factor to stimulate proliferation. The ET-1-mediated cell proliferation was inhibited in the presence of U0126, a specific inhibitor of (mitogen-activated protein kinase ERK kinase), indicating that extracellular signal-regulated kinase (ERK) activation is involved. Additionally, ET-1 induced the activation of phospholipase (PL) D, leading to the synthesis of phosphatidic acid (PA). The ET-1-induced activation of PLD was twofold higher in ELT-3 cells compared to that in normal cells. The two cell types expressed mRNA for PLD1a and PLD2, whereas PLD1b was expressed only in ELT-3 cells. The exposure of cells to butan-1-ol reduced ET-1-mediated production of PA by PLD and partially inhibited ERK activat...Continue Reading

References

Oct 1, 1990·American Journal of Clinical Pathology·S F Cramer, A Patel
Oct 1, 1981·Fertility and Sterility·V C Buttram, R C Reiter
Aug 1, 1996·Seminars in Reproductive Endocrinology·J Andersen
Oct 8, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P M SteedD J Lasala
May 31, 2001·The Journal of Biological Chemistry·J D PaascheH Attramadal
Nov 1, 2001·The Journal of Biological Chemistry·L A Denmat-OuisseM N Raymond
Dec 26, 2001·Biology of Reproduction·Isabelle EudeMichelle Breuiller-Fouché
May 4, 2002·Reviews of Physiology, Biochemistry and Pharmacology·J H Exton
Jun 11, 2002·American Journal of Physiology. Cell Physiology·Philippe RobinDenis Leiber
Jun 14, 2002·The Journal of Biological Chemistry·Hirofumi SonodaHiroyuki Arai
Feb 4, 2003·Nature Reviews. Cancer·Joel NelsonPerry Nisen
Apr 18, 2003·American Journal of Physiology. Cell Physiology·G A FinlayB L Fanburg
May 16, 2003·American Journal of Physiology. Cell Physiology·G Di LibertoM Breuiller-Fouché
Aug 2, 2003·Nature Reviews. Cancer·Gordon B Mills, Wouter H Moolenaar
Oct 21, 2003·Genes, Chromosomes & Cancer·Cheryl Lyn WalkerJeffery I Everitt
Dec 3, 2003·American Journal of Physiology. Cell Physiology·Philippe RobinDenis Leiber
Jan 14, 2004·Molecular Biology of the Cell·Guangwei DuMichael A Frohman

❮ Previous
Next ❯

Citations

Jul 20, 2005·Neoplasia : an International Journal for Oncology Research·Joel B NelsonBeth R Pflug
Mar 1, 2006·Obstetrics and Gynecology Clinics of North America·Ibrahim Sozen, Aydin Arici
Oct 29, 2013·Obstetrics and Gynecology International·Andrea CiavattiniPasquapina Ciarmela
Oct 27, 2010·The International Journal of Biochemistry & Cell Biology·Zahra TanfinMichelle Breuiller-Fouché
Apr 6, 2013·Fertility and Sterility·Md Soriful IslamPasquapina Ciarmela
Aug 5, 2016·Applied Microbiology and Biotechnology·Catherine EsnaultMarie-Joelle Virolle
May 4, 2012·Biology of Reproduction·Zahra Tanfin, Michelle Breuiller-Fouché

❮ Previous
Next ❯

Methods Mentioned

BETA
FCS
scraping
PCR
electrophoresis
reverse transcription-PCR

Related Concepts

Related Feeds

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.