Contribution of postnatally formed small beta cell aggregates to functional beta cell mass in adult rat pancreas.

Diabetologia
Marie ChintinneDaniel Pipeleers

Abstract

Neogenesis of beta cells and their clustering to small aggregates is a key process in prenatal development of beta cell mass. We investigated the contribution of postnatally formed small aggregates to functional beta cell mass in adult rats. Conditions were defined for (1) counting total beta cell number in pancreases with relative error of <10% and (2) determining their distribution over aggregates of different size and over functionally different subpopulations. Pancreases of 10-week-old male Wistar rats contained 2.8 ± 0.2 × 10⁶ beta cells, of which >90% was generated postnatally, involving: (1) neo-formation of 30,000 aggregates with diameter <50 μm including single cells; and (2) growth of 5,500 aggregates to larger sizes, accounting for 90% of the increase in cell number, with number of growing aggregates in the tail 50% greater than elsewhere. At 10 weeks, 86% of aggregates were <50 μm; compared with aggregates >200 μm, their beta cells exhibited a higher basal insulin content that was also resistant to glibenclamide-induced degranulation. The pool of Ki67-positive beta cells was sixfold larger than at birth and distributed over all aggregate sizes. We describe a method for in situ counting of beta cell numbers and subpo...Continue Reading

References

Jul 1, 1992·Diabetes·D G Pipeleers
Jul 1, 1987·The Journal of Clinical Investigation·Y StefanL Orci
May 1, 1983·Diabetologia·J RahierJ C Henquin
May 29, 1999·Journal of Microscopy·H J Gundersen Nielsen J
Oct 9, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·T BockK Buschard
Mar 29, 2001·Diabetes·S Bonner-Weir
Jun 22, 2002·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Kirsten SvenstrupTroels Bock
Dec 28, 2002·Diabetes·Alexandra E ButlerPeter C Butler
Nov 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Bart KeymeulenDaniel Pipeleers
Sep 8, 2007·The Journal of Clinical Investigation·Lingwen ZhongAnil Bhushan
Oct 18, 2008·Diabetes, Obesity & Metabolism·J RahierJ C Henquin
Oct 18, 2008·Diabetes, Obesity & Metabolism·D PipeleersF Gorus
Dec 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Akari InadaSusan Bonner-Weir
Feb 1, 1946·The Anatomical Record·M ABERCROMBIE

❮ Previous
Next ❯

Citations

Mar 23, 2012·American Journal of Physiology. Endocrinology and Metabolism·Adriana Rodríguez-TrejoMarta Menjivar
Jan 1, 2012·The Review of Diabetic Studies : RDS·Susan Bonner-WeirArun Sharma
Dec 31, 2010·Diabetologia·C Aguayo-MazzucatoS Bonner-Weir
Feb 1, 2013·Annals of the New York Academy of Sciences·Gordon C Weir, Susan Bonner-Weir
Mar 22, 2016·Upsala Journal of Medical Sciences·Susan Bonner-WeirGordon C Weir
Mar 30, 2017·The Journal of Endocrinology·Christine A BeamishDavid J Hill
Jul 29, 2015·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Daniel T MeierSteven E Kahn
Aug 20, 2016·Therapeutic Advances in Endocrinology and Metabolism·Elisa CorritorePhilippe A Lysy
Sep 21, 2013·Journal of Molecular Endocrinology·G A MartensD Pipeleers
Feb 16, 2019·Endocrine Connections·Caio Jordão TeixeiraSilvana Bordin
Apr 25, 2014·American Journal of Physiology. Endocrinology and Metabolism·Maria L GolsonMarcela Brissova
Sep 8, 2020·Diabetologia·Cristina Aguayo-Mazzucato
Aug 5, 2017·Genesis : the Journal of Genetics and Development·Matthew E BechardChristopher V E Wright
Mar 28, 2013·American Journal of Physiology. Endocrinology and Metabolism·Martín CrivelloVictoria Lux-Lantos
Apr 30, 2021·Experimental Physiology·Catharina de Barros Pimentel VillaçaFernanda Ortis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.