PMID: 8938543Nov 1, 1996Paper

Contribution of primary cultures of adult human hepatocytes to the pathophysiology of hepatocellular carcinoma

Journal of Hepatology
P BlancP Maurel

Abstract

The mechanisms of hepatocarcinogenesis are still poorly understood. The development of hepatocellular carcinoma has recently been shown to be associated with increased DNA synthesis in cirrhosis. The aim of this work was to determine whether the high rate of hepatocyte regeneration observed in cirrhotic liver with hepatocellular carcinoma is associated with the presence of a growth factor that could be detectable in the serum. Adult human hepatocytes in primary culture, allowing the evaluation of the release of circulating hepatotrophic factors, were used. These cultures were treated for 48 h with serum from patients with cirrhosis with and without hepatocellular carcinoma, from patients with liver metastasis, and from healthy subjects. The rate of DNA synthesis in these cultures was assessed by measuring the amount of [3H]-thymidine incorporation into genomic DNA. On average, the synthesis of DNA was increased 2.5-, 2.2-, 2.1-, and 2.3-fold, respectively, in response to serum from patients with cirrhosis with hepatocellular carcinoma, from patients with cirrhosis without hepatocellular carcinoma, from patients with liver metastasis, and from healthy subjects. We conclude that the hepatotrophic activity of the serum is not sign...Continue Reading

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Citations

Jun 12, 2012·BMC Gastroenterology·Spiros P HiotisHerman T Yee
Feb 21, 2004·Clinical Endocrinology·Daniele MatteraLuciano D'Agostino

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