Abstract
The mechanisms of hepatocarcinogenesis are still poorly understood. The development of hepatocellular carcinoma has recently been shown to be associated with increased DNA synthesis in cirrhosis. The aim of this work was to determine whether the high rate of hepatocyte regeneration observed in cirrhotic liver with hepatocellular carcinoma is associated with the presence of a growth factor that could be detectable in the serum. Adult human hepatocytes in primary culture, allowing the evaluation of the release of circulating hepatotrophic factors, were used. These cultures were treated for 48 h with serum from patients with cirrhosis with and without hepatocellular carcinoma, from patients with liver metastasis, and from healthy subjects. The rate of DNA synthesis in these cultures was assessed by measuring the amount of [3H]-thymidine incorporation into genomic DNA. On average, the synthesis of DNA was increased 2.5-, 2.2-, 2.1-, and 2.3-fold, respectively, in response to serum from patients with cirrhosis with hepatocellular carcinoma, from patients with cirrhosis without hepatocellular carcinoma, from patients with liver metastasis, and from healthy subjects. We conclude that the hepatotrophic activity of the serum is not sign...Continue Reading
References
Nov 1, 1977·Analytical Biochemistry·J Kapuściński, B Skoczylas
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·G ShiotaE V Schmidt
May 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K Okuda
Nov 1, 1991·Japanese Journal of Cancer Research : Gann·Y ShimoyamaS Hirohashi
Jan 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H TsubouchiS Kawakami
May 1, 1991·The Journal of Clinical Investigation·A J StrainP McMaster
Feb 15, 1991·Science·D P BottaroS A Aaronson
Dec 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T IsmailA J Strain
Jul 1, 1991·Digestive Diseases and Sciences·E Farber
Mar 1, 1988·Annals of Internal Medicine·A M Di BisceglieM T Lotze
May 1, 1988·American Journal of Surgery·C H ScudamoreD A Owen
Jun 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H TsubouchiY Muto
Sep 15, 1989·Biochemical and Biophysical Research Communications·K MiyazawaK Takahashi
Jul 1, 1989·Cancer·K TaraoT Okamoto
Dec 1, 1988·Journal of Hepatology·J J Berman
Feb 1, 1987·Journal of Hepatology·P J Johnson, R Williams
Feb 1, 1988·The Journal of Clinical Investigation·E GohdaY Daikuhara
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T NakamuraA Ichihara
Oct 19, 1973·Nature·V M Craddock
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H C Isom, I Georgoff
Sep 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P BlancP Maurel