PMID: 11312149Apr 20, 2001Paper

Contribution of progesterone, follicle stimulating hormone and glucocorticoids in survival of serum-free cultured granulosa cell explants

The Journal of Endocrinology
S Mussche, K D'Herde

Abstract

To investigate the role of progesterone (P4) as a survival factor in quail granulosa cell explants, P4 content was determined under various conditions and correlated with apoptotic indexes (AIs) evaluated by 2',6'-diamidino-2-phenylindole (DAPI)-staining. Analysis of serum-free cultures from 24 to 96 h shows decreased P4 levels in the medium paralleled by increasing AI. Inhibiting apoptosis by gonadotropic support (FSH, 100 ng/ml) stimulates a 3-fold increase of the P4 level in the medium (83.49+/-8.69 vs 26.31+/-1.61 ng/ml in serum-free controls) together with a significant decrease in AI from 8.81+/-1.06% in serum-free controls to 3.50+/-0.72%. Substantial evidence for P4 as an autocrine/paracrine survival factor can be inferred from experiments with aminoglutethimide (AG, 1 mM) and RU486 (20 microM). Blocking P4 synthesis by AG causes a 2-fold increase in apoptosis from 6.08+/-0.67% in serum-free controls to 12.53+/-1.60%. Blocking P4 receptors by RU486 causes a similar increase in AI (3.02+/-0.98% in serum-free controls to 17.07+/-3.20%) and about a 50% decrease in P4. The effect of RU486 could be attenuated by exogenous P4 but not by dexamethasone indicating selective binding of P4 to the progesterone receptor. Dexamethaso...Continue Reading

Citations

Jul 16, 2008·Apoptosis : an International Journal on Programmed Cell Death·Dmitri V KryskoKatharina D'Herde
Jun 19, 2002·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Christophe SiferGerard Feldmann
Aug 23, 2003·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Christophe SiferJean-Louis Benifla
Apr 30, 2005·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Jennifer A LemonC David Rollo
Aug 21, 2003·Archives of Gynecology and Obstetrics·Jochen ReinsbergHans van der Ven
Feb 27, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Araceli Diez-FraileKatharina G M A D'Herde
Jun 17, 2006·Gynécologie, obstétrique & fertilité·G FeldmannP Madelenat
Dec 13, 2005·Molecular and Cellular Endocrinology·Moshood O OlatinwoDavid R Mann

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