PMID: 9540834Apr 16, 1998Paper

Contribution of proximal promoter elements to the regulation of basal and differential glutathione S-transferase P1 gene expression in human breast cancer cells

Biochimica Et Biophysica Acta
M S Jhaveri, C S Morrow

Abstract

Glutathione S-transferase P1 (GST P1-1) is normally expressed exclusively in estrogen receptor negative (ER-) but not receptor positive (ER+) cultured breast cancer cells. We examined the role of proximal promoter elements in GST P1 gene expression in MCF7 (ER+, GST P1-) and HS578T (ER-, GST P1+) breast cancer cells. Transient transfection of GST P1 promoter-CAT reporter genes confirmed that the GST P1 TRE (-69 to -60) and the adjacent distal GC box (-56 to -51) are required for basal promoter activity in both cell lines. Other studies identified differences in the GST P1 promoter activity and DNA-protein interactions between the two cell lines. Electrophoretic mobility shift assay revealed a protein-TRE interaction that is unique to nuclear proteins derived from GST P1 expressing HS578T cells. Furthermore, a putative silencer region contained within sequences -130 to -70 selectively reduced GST P1 promoter-CAT reporter gene expression in MCF7 but not HS578T cells. While this cell-line specific silencer contributed to the level of GST P1 promoter activity observed in the two cell lines, analysis of cells stably transfected with a novel genomic GST P1 minigene vector established that the silencer is insufficient to completely re...Continue Reading

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Citations

Aug 29, 2012·Research in Veterinary Science·M GiantinM Dacasto
Jan 7, 2016·Environment International·Ling LengNai-Jun Tang
Sep 22, 2005·Endocrine Reviews·J KöhrleJ E Dumont
May 8, 2004·Endocrinology·Anne Marie VelascoYixian Zhang
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Mar 21, 2012·Epigenetics : Official Journal of the DNA Methylation Society·Breezy Malakkaran LindqvistTorbjörn K Nilsson
Jan 8, 2014·Molecular Nutrition & Food Research·Emmanuel AnsongAlan M Diamond
Jul 16, 2014·Molecular Medicine Reports·Weihan YangWen Zhu
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Dec 5, 2014·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Petra MatouškováLenka Skálová
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Aug 1, 2002·Japanese Journal of Cancer Research : Gann·Noboru KonishiKeiji Shimada
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Jun 9, 2020·The Prostate·Mostafa ElhodakyAlan M Diamond
Aug 10, 2004·Alimentary Pharmacology & Therapeutics·T Y KimY-J Bang

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