Contribution of quorum-sensing system to hexadecane degradation and biofilm formation in Acinetobacter sp. strain DR1

Journal of Applied Microbiology
Y-S Kang, Woojun Park

Abstract

To investigate roles of quorum-sensing (QS) system in Acinetobacter sp. strain DR1 and rifampicin-resistant variant (hereinafter DR1R). The DR1 strain generated three putative acyl homoserine lactones (AHLs), while the DR1R produced only one signal and QS signal production was abrogated in the aqsI (LuxI homolog) mutant. The hexadecane-degradation and biofilm-formation capabilities of DR1, DR1R, and aqsI mutants were compared, along with their proteomic data. Proteomics analysis revealed that the AHL lactonase responsible for degrading QS signal was highly upregulated in both DR1R and aqsI mutant, also showed that several proteins, including ppGpp synthase, histidine kinase sensors, might be under the control of QS signalling. Interestingly, biofilm-formation and hexadecane-biodegradation abilities were reduced more profoundly in the aqsI mutant. These altered phenotypes of the aqsI mutant were restored via the addition of free wild-type cell supernatant and exogenous C(12) -AHL. The QS system in strain DR1 contributes to hexadecane degradation and biofilm formation. This is the first report to demonstrate that a specific QS signal appears to be a critical factor for hexadecane degradation and biofilm formation in Acinetobacter...Continue Reading

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Citations

May 4, 2011·The Journal of Microbiology·Jaejoon JungWoojun Park
Jul 20, 2010·Journal of Bacteriology·Jaejoon JungWoojun Park
May 20, 2014·Archives of Microbiology·Madhvi N JoshiSnehal B Bagatharia
Jan 17, 2012·Proteomics·C Jayampath SeneviratneLakshman P Samaranayake
May 19, 2019·Journal of Applied Microbiology·K SaipriyaV Sritharan
Jul 6, 2019·Frontiers in Microbiology·Yao Lei ZhuHong Shun Hao
Oct 3, 2018·Frontiers in Cellular and Infection Microbiology·Celia MayerAna Otero
Jan 5, 2020·Applied Microbiology and Biotechnology·Bora ShinWoojun Park
Jan 27, 2021·Journal of Bacteriology·Mario López-MartínPaul Williams

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