Contribution of selectins to leucocyte sequestration in pulmonary microvessels by intravital microscopy in rabbits

The Journal of Physiology
W M KueblerA E Goetz

Abstract

1. Sequestration of leucocytes in the lung is the net result of leucocyte rolling and sticking in pulmonary arterioles and venules and their retention in alveolar capillaries. 2. In order to investigate whether adhesion molecules of the selectin family contribute to these phenomena the effects of fucoidin (an inhibitor of L- and P-selectin) on microhaemodynamics and leucocyte kinetic were studied in pulmonary arterioles, capillaries and venules by means of intravital fluorescence microscopy in a rabbit model. 3. Fucoidin reduced leucocyte rolling in pulmonary arterioles and venules by 75 and 83%, respectively, without affecting leucocyte sticking. In alveolar capillaries, fucoidin reduced leucocyte retention and accelerated leucocyte passage, thus reducing the alveolar transit time of leucocytes by 62%. 4. It is concluded that rolling of leucocytes in pulmonary microvessels is mediated by selectins, whereas sticking relies on selectin-independent mechanisms. 5. Leucocyte retention in alveolar capillaries is not due solely to mechanical hindrance of leucocyte passage through narrow vessel segments, as previously hypothesized, but also depends on interaction of leucocytes with the capillary endothelium.

References

Dec 11, 1975·Journal of Applied Physiology·W W Wagner, L P Latham
Oct 1, 1992·The Journal of Clinical Investigation·M S MulliganP A Ward
Dec 31, 1991·Biochemical and Biophysical Research Communications·K HandaS Hakomori
Aug 2, 1990·Nature·T A Springer
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T K KishimotoE C Butcher
Sep 1, 1988·Anaesthesia·K G LjungströmB E Wiholm
Sep 1, 1988·Journal of Applied Physiology·J C HoggB Wiggs
Nov 1, 1987·Journal of Applied Physiology·C M DoerschukJ C Hogg
Jan 1, 1982·International Archives of Allergy and Applied Immunology·H Hedin, W Richter
Aug 1, 1982·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·W G GuntherothI Kawabori
Nov 1, 1980·Microvascular Research·H N MayrovitzM P Wiedeman
Jul 1, 1994·Kidney International·P G TippingS R Holdsworth
Jun 1, 1995·The Journal of Clinical Investigation·P KubesD Payne
Oct 1, 1995·American Journal of Respiratory and Critical Care Medicine·G E KuhnleA E Goetz
Aug 1, 1995·Journal of Applied Physiology·S A GebbW W Wagner
Jan 1, 1994·Journal of Applied Physiology·W M KueblerA E Goetz
Sep 1, 1963·Journal of Applied Physiology·S PERMUTT, R L RILEY

❮ Previous
Next ❯

Citations

Oct 24, 2013·Environmental Toxicology and Pharmacology·Ara ChoBumseok Kim
Jun 5, 2003·Burns : Journal of the International Society for Burn Injuries·Cengiz CetinYakup Karabağli
May 4, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Chun ZhouSongwei Wu
Jan 5, 2000·American Journal of Respiratory and Critical Care Medicine·W M KueblerA E Goetz
May 6, 2003·American Journal of Respiratory Cell and Molecular Biology·Susan M GerikFrank C Schmalstieg
Jan 24, 2003·American Journal of Respiratory Cell and Molecular Biology·Sunita BhattacharyaJahar Bhattacharya
Aug 17, 1999·The Journal of Clinical Investigation·W M KueblerJ Bhattacharya
May 10, 2000·American Journal of Veterinary Research·D McClenahanD Weiss
May 9, 2002·The Annals of Thoracic Surgery·Adrian J LevineRobert S Bonser
May 14, 2004·Thrombosis Research·Victor L SerebruanyCharles H Hennekens
Jul 20, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sharmila ChoudhuryMasao Takata
Aug 24, 2006·Journal of Vascular Research·Rainer KiefmannAlwin Eduard Goetz
Nov 11, 2006·Journal of Applied Physiology·Wolfgang M KueblerJahar Bhattacharya
Mar 18, 2003·Journal of Applied Physiology·Andreas J SchwabJocelyn Dupuis
Dec 29, 2018·American Journal of Respiratory and Critical Care Medicine·Prithu Sundd, Wolfgang M Kuebler
Feb 6, 2020·International Journal of Molecular Sciences·Vincent D GiacaloneRabindra Tirouvanziam
Jan 23, 2013·Immunology and Cell Biology·Michael J Hickey, Clare L V Westhorpe
Mar 29, 2003·Physiological Reviews·Alan R BurnsDavid C Walker
Nov 13, 2018·Frontiers in Cardiovascular Medicine·Nikita P PatilAaron B Baker
Apr 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Wolfgang M KueblerJahar Bhattacharya
Jun 10, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Lyudmila SikoraP Sriramarao
Apr 21, 2020·Frontiers in Physiology·Jasmin MatuszakWolfgang M Kuebler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.