Contribution of surface histidyl residues in the alpha-chain to the Bohr effect of human normal adult hemoglobin: roles of global electrostatic effects

Biochemistry
D P SunC Ho

Abstract

We have applied site-directed mutagenesis to our Escherichia coli hemoglobin expression plasmid and constructed five recombinant mutant hemoglobins (r Hbs): r Hb(alpha20His-->Gln or alpha:H20Q); r Hb(alpha:H50Q); r Hb(alpha:H72Q); r Hb(alpha:H89Q); and r Hb(alpha:H112Q). We have constructed these r Hbs to help us assess the contribution of the surface histidyl residues in the alpha-chain to the alkaline Bohr effect of human normal adult hemoglobin (Hb A). In our laboratory, we have monitored the variation of proton nuclear magnetic resonances arising from the C2 protons of the histidyl residues of Hb A as a function of pH and buffer conditions. Several of these resonances have been assigned to the individual histidyl residues on the surface of the hemoglobin molecule using naturally occurring mutant hemoglobins and chemically modified hemoglobins. In the present work, we have identified the C2 proton resonances of five surface histidyl residues of the alpha-chain, alpha20, alpha50, alpha72, alpha89, and alpha112, in both the carbonmonoxy and deoxy forms, by comparing the proton nuclear magnetic resonance spectra of Hb A with those of the r Hbs. For the assignment of the C2 proton resonances of alpha20His and alpha112His, we hav...Continue Reading

Citations

Apr 17, 2007·Journal of Biosciences·S Rezaei-ZarchiA Javed
Jul 20, 2002·Biophysical Chemistry·Ching-Hsuan Tsai, Chien Ho
Nov 3, 2010·Acta Crystallographica. Section D, Biological Crystallography·Andrey KovalevskyYukio Morimoto
Nov 3, 2010·Acta Crystallographica. Section D, Biological Crystallography·Andrey KovalevskyPaul Langan
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Jun 24, 2015·Journal of Theoretical Biology·Kehinde O Okonjo
Jan 22, 2015·Chemical Reviews·Yue YuanChien Ho
Oct 23, 2016·Journal of Theoretical Biology·Kehinde O Okonjo
Dec 22, 2007·Journal of Applied Physiology·Babs R SollerR Donald Hagan
Mar 11, 2004·Chemical Reviews·Jonathan A Lukin, Chien Ho

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