PMID: 8943384Dec 1, 1996Paper

Contribution of the CD28 molecule to allergic and irritant-induced skin reactions in CD28 -/- mice

The Journal of Immunology : Official Journal of the American Association of Immunologists
S KondoD N Sauder

Abstract

The CD28 molecule is thought to be essential for the costimulatory signals and is required for mitogenic activation of T cells. The aim of this study was to evaluate the contribution of CD28 in contact hypersensitivity using CD28 gene-targeted (CD28 -/-) mutant mice. Contact hypersensitivity to dinitrofluorobenzene and oxazolone was significantly decreased in CD28 -/- mice compared with that in normal (C57BL/6) mice. Significant increases in IL-2 mRNA were detected in the skin after dinitrofluorobenzene challenge in normal mice, while this response was blunted in CD28 -/- mice. In addition, responses to irritant chemicals (croton oil and phenol) were also impaired in CD28 -/- mice. IL-2, IFN-gamma, and TNF-alpha mRNA levels were lower in CD28 -/- mouse skin treated with phenol. T cells from CD28 -/- mice did not bind to epidermal cells derived from normal mice, while cocultivation of T cells with epidermal cells from normal mice demonstrated a significant binding. These results indicate that the CD28 molecule is required not only for optimal induction of allergic contact dermatitis, but also for the development of irritant-induced skin inflammation. The expression of B7-1 and B7-2 molecules on epidermal cells was induced by an ...Continue Reading

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