Contribution of the endoplasmic reticulum to the glucose-induced [Ca(2+)](c) response in mouse pancreatic islets

American Journal of Physiology. Endocrinology and Metabolism
Abdelilah ArredouaniP Gilon

Abstract

Thapsigargin (TG), a blocker of Ca(2+) uptake by the endoplasmic reticulum (ER), was used to evaluate the contribution of the organelle to the oscillations of cytosolic Ca(2+) concentration ([Ca(2+)](c)) induced by repetitive Ca(2+) influx in mouse pancreatic beta-cells. Because TG depolarized the plasma membrane in the presence of glucose alone, extracellular K(+) was alternated between 10 and 30 mM in the presence of diazoxide to impose membrane potential (MP) oscillations. In control islets, pulses of K(+), mimicking regular MP oscillations elicited by 10 mM glucose, induced [Ca(2+)](c) oscillations whose nadir remained higher than basal [Ca(2+)](c). Increasing the depolarization phase of the pulses while keeping their frequency constant (to mimic the effects of a further rise of the glucose concentration on MP) caused an upward shift of the nadir of [Ca(2+)](c) oscillations that was reproduced by raising extracellular Ca(2+) (to increase Ca(2+) influx) without changing the pulse protocol. In TG-pretreated islets, the imposed [Ca(2+)](c) oscillations were of much larger amplitude than in control islets and occurred on basal levels. During intermittent trains of depolarizations, control islets displayed mixed [Ca(2+)](c) osci...Continue Reading

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Citations

Sep 23, 2014·Cell Calcium·Patrick GilonMagalie A Ravier
Feb 24, 2009·Nature Chemical Biology·Edmund NaylorGrant C Churchill
Jul 16, 2010·American Journal of Physiology. Endocrinology and Metabolism·L E FridlyandL H Philipson
Jul 24, 2010·Endocrine Reviews·Stanko S StojilkovicRichard Bertram
Feb 2, 2008·Bulletin of Mathematical Biology·Richard Bertram, Rudy C Arceo
Sep 1, 2004·Biochemical and Biophysical Research Communications·Béla PappChristine Chomienne
May 26, 2010·Theoretical Biology & Medical Modelling·Leonid E Fridlyand, Louis H Philipson
Sep 2, 2016·Physiological Reviews·David G Nicholls
Sep 21, 2017·Science Signaling·Nicholas C VierraDavid A Jacobson
Dec 5, 2019·Endocrinology·Irina X ZhangLeslie S Satin
Jan 24, 2014·American Journal of Physiology. Endocrinology and Metabolism·M BelzI Rustenbeck
May 3, 2012·American Journal of Physiology. Endocrinology and Metabolism·M WillenborgI Rustenbeck

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