Contribution of the Na(+)-K(+)-2Cl(-) cotransporter (NKCC1) to transepithelial transport of H(+), NH(4)(+), K(+), and Na(+) in rat outer medullary collecting duct.

Journal of the American Society of Nephrology : JASN
S M Wall, Michael P Fischer

Abstract

In rat kidney, the "secretory" isoform of the Na-K-Cl cotransporter, NKCC1 (BSC-2), localizes to the basolateral membrane of the alpha intercalated cell, the acid secreting cell of the outer medullary collecting duct (OMCD). This laboratory has reported that NKCC1 mediates Cl(-) uptake across the basolateral membrane in series with Cl(-) secretion across the apical membrane in rat OMCD. NKCC1 transports NH(4)(+), K(+), and Na(+) as well as Cl(-); therefore, a role for the cotransporter in the process of HCl, NH(4)Cl, KCl, and NaCl secretion has been suggested. Thus, it was determined if bumetanide, an inhibitor of NKCC1, alters transepithelial cation transport in rat OMCD. OMCD tubules from deoxycorticosterone pivalate (DOCP)-treated rats were perfused in vitro. Hydration of CO(2), rather than NH(4)(+), provides the principle source of H(+) for net acid secretion. In HCO(3)(-)/CO(2)-buffered solutions, no effect of bumetanide on net K(+) flux was detected. Under some conditions, bumetanide addition resulted in a small reduction in secretion of net H(+) equivalents. Transepithelial Na(+) flux, J(Na), was -1.5 +/- 1.7 pmol/mm per min, values not different from zero. However, with the application of bumetanide to the bath, J(Na) w...Continue Reading

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Citations

Aug 10, 2004·Current Opinion in Nephrology and Hypertension·I David Weiner
Dec 6, 2003·Acta Physiologica Scandinavica·S M Wall
Jul 28, 2005·Current Opinion in Nephrology and Hypertension·Susan M Wall
Aug 4, 2005·American Journal of Physiology. Renal Physiology·Régine ChambreyDominique Eladari
Sep 15, 2005·American Journal of Physiology. Renal Physiology·Susan M WallYoung-Hee Kim
Mar 28, 2006·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·I D Weiner
Oct 31, 2006·Seminars in Nephrology·Patricia VallesDaniel Batlle
Sep 28, 2006·Annual Review of Physiology·I David Weiner, L Lee Hamm
Mar 12, 2009·Pflügers Archiv : European journal of physiology·Carsten A WagnerNilufar Mohebbi
Apr 25, 2009·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Shiho WakamatsuKimio Tomita
Oct 19, 2020·Journal of Molecular Histology·Zinaeli López-GonzálezLaura I Escobar
Apr 30, 2010·American Journal of Physiology. Renal Physiology·Rolando Carrisoza-GaytánLaura I Escobar
Nov 5, 2010·American Journal of Physiology. Renal Physiology·I David Weiner, Jill W Verlander
Jul 29, 2011·American Journal of Physiology. Renal Physiology·Shaikh Abu HossainHassane Amlal
Jul 29, 2011·Kidney International·Rolando Carrisoza-GaytánLaura I Escobar
Aug 6, 2011·American Journal of Physiology. Renal Physiology·Wen LiuLisa M Satlin
Dec 22, 2020·Function·Rainelli KoumangoyeEric Delpire

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