Contribution of the resonance Raman spectroscopy to the identification of Z DNA

Journal of Biomolecular Structure & Dynamics
B JollèsA Laigle

Abstract

Poly(dG-dC).poly(dG-dC) at low salt concentration (0.1 M NaCl) and at high salt concentration (4.5 M NaCl) has been studied by Raman resonance spectroscopy using two excitation wavelengths: 257 nm and 295 nm. As resonance enhances the intensity of the lines in a proportion corresponding to the square of the molar absorption coefficient, the intensities of the lines with 295 nm wavelength excitation are enhanced about sevenfold during the B to Z transition. With 257 nm excitation wavelength the 1580 cm-1 line of guanosine is greatly enhanced in the Z form whereas with 295 nm excitation several lines are sensitive to the modifications of the conformation: the guanine band around 650 cm-1 and at 1193 cm-1 and the bands of the cytosines at 780 cm-1, 1242 cm-1 and 1268 cm-1. By comparison with the U.V. resonance Raman spectra of DNA, we conclude that resonance Raman spectroscopy allows one to characterize the B to Z transition from one line with 257 nm excitation wavelength and from three lines with 295 nm excitation. The conjoined study of these four lines should permit to observe a few base pairs being in Z form in a DNA.

References

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Citations

Mar 4, 2000·Eye·S M RecuperoS Calvieri
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·W L KubasekW L Peticolas
Dec 1, 1993·Journal of Biomolecular Structure & Dynamics·P MiskovskyP Y Turpin
Feb 1, 1995·Journal of Biomolecular Structure & Dynamics·V BerthelierL Chinsky
Feb 1, 1997·Journal of Biomolecular Structure & Dynamics·V LisyL Chinsky
Apr 12, 2013·Cancer Biotherapy & Radiopharmaceuticals·Mu-hua ChengLiang-jun Xie
Apr 11, 1989·Nucleic Acids Research·A LaigleB Jollès
Apr 1, 1988·Photochemistry and Photobiology·D T CrokeD J McConnell
Dec 1, 1995·Journal of Biomolecular Structure & Dynamics·P MiskovskyP Y Turpin
Dec 1, 1989·Journal of Biomolecular Structure & Dynamics·P MiskovskyP Y Turpin
Aug 21, 1985·Biochimica Et Biophysica Acta·M Leng

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